dig labeling
|
DIG DNA Labeling and Detection Kit
DIG DNA Labeling and Detection Kit Control DNA 1 unlabeled Clear solution pBR328 100 μg/ml digested separately with Bam Hl Bgl l and Hinf l Digests are combined in a ratio of 2:3:3 16 pBR328 fragment sizes: 4907 2176 1766 1230 1033 653 517 453 394 298 (2 ×) 220 and 154 (2 ×) bp |
What is dig DNA labeling & detection kit?
The DIG DNA Labeling and Detection Kit uses digoxigenin (DIG), a steroid hapten to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. DIG-labeled DNA probes are generated according to the random primed labeling technique.
What is dig labeling?
DIG labeling supports posthybridization detection by chemiluminescence or by formation of an insoluble color precipitate directly on the filter membrane. This method is also well respected for in situ hybridization applications ( Robbins et al., 1991; Crabb et al., 1992; Schröder et al., 2000 ).
What is a digoxigenin labeling system?
The digoxigenin–anti-digoxigenin labeling system uses digoxigenin (DIG), a cardenolide steroid isolated from Digitalis plants (Martin et al., 1990 ). A nucleotide triphosphate analog containing the digoxigenin moiety ( Figure 3.3) is incorporated into DNA by nick translation or random primer labeling.
Does dig labeling interfere with biotinylation?
As with biotinylation, DIG labeling does not interfere with biological activity, and probes so labeled are stable at –20° for at least 1 year after labeling. DIG labeling supports posthybridization detection by chemiluminescence or by formation of an insoluble color precipitate directly on the filter membrane.
IV. PROTOCOLS FOR DIGOXIGENIN LABELING AND DETECTION OF NUCLEIC ACIDS
In the following paragraphs we describe the essential steps of labeling DNA and RNA probes with digoxigenin and give protocols for detection of DIG labeled hybrids in tissue sections and on nylon filters. The hybridization and washing steps in individual applications are often very similar to those used with radiolabeled probes, hence they are not
0/GOX/GEN/N LABELLED PROBES 109
precipitated and the pellet is washed with 70% ethanol, dried and resuspended in diethyl-pyrocarbonate-water or TE-buffer. RNase inhibitor from the kit is added to inhibit possible contaminating RNases. An aliquot of the transcript is analyzed by agarose gel electrophoresis and ethidium bromide staining or by subsequent blotting and incubation of t
P. HEINO et of.
Traditionally, radiolabeled probes are widely used in nucleic acid hybridization protocols. Handling of radioactive materials requires special safety measures and working spaces. Considering this and the short shelf life of radiolabeled probes, the nonisotopic labeling systems have been met with great interest. The most commonly used nonisotopic la
|
DIG RNA Labeling Mix
RNA labeling with digoxigenin-UTP by in vitro transcription with SP6 T7 |
|
DIG RNA Labeling Kit (SP6/T7)
Use the DIG RNA Labeling Kit (SP6/T7) to label RNA with digoxigenin-UTP by in vitro transcription. DIG-labeled RNA is used for hybridization to: • Northern |
|
DIG DNA Labeling Kit
DIG-labeled DNA probes can be used in a variety of applications: • All types of filter hybridization according to our standard protocol in the Instructions for |
|
PCR DIG Labeling Mix
chain reactions (1) and the DIG-(digoxigenin-) labeled nucleotide will be incorporated into the PCR product (2). Taq DNA polymerase as well as Tth DNA. |
|
The DIG System - Labeling and Detection of Nucleic Acids
DIG labeling produces high sensitivity and low background. Easily label. - DNA. - RNA. - oligonucleotides. Label the probe using familiar efficient procedures. |
|
DIG DNA Labeling and Detection Kit
DIG-labeled DNA probes are generated according to the method of random primed labeling [1] which is based on the hybridization of random oligonucleotides to |
|
DIG Northern Starter Kit
For transcription labeling of RNA with digoxigenin and SP6/T7/T3 RNA Polymerases and For the hybridization of the DIG-labeled RNA probe. 2 bottles. |
|
DIG High Prime DNA Labeling and Detection Starter Kit I
(DIG) a steroid hapten |
|
DIG DNA Labeling Mix
DIG-labeled DNA probes are generated according to the method of random primed labeling which is based on the hybridization of random oligonucleotides to the |
|
For Nonradioactive In Situ Hybridization
RNA-RNA in situ hybridization using DIG-labeled probes: the effect of high molecular weight polyvinyl alcohol on the alkaline phosphatase indoxyl-nitroblue |
|
DIG DNA Labeling and Detection Kit - Sigma-Aldrich
DIG-labeled DNA probes are generated according to the method of random primed labeling [1] which is based on the hybridization of random oligonucleotides |
|
PCR DIG Labeling Mix - Sigma-Aldrich
chain reactions (1) and the DIG-(digoxigenin-) labeled nucleotide will be incorporated into the PCR product (2) Taq DNA polymerase, as well as Tth DNA |
|
I In situ hybridization with DIG-labeled probes - Roche Life Science
RNA-RNA in situ hybridization using DIG-labeled probes: the effect of high molecular weight polyvinyl alcohol on the alkaline phosphatase indoxyl-nitroblue |
|
DIG DNA Labeling Kit - BRIC
DIG DNA Labeling Kit Random primed DNA labeling with digoxigenin-dUTP, alkali-labile Cat No 11 175 033 910 Version October 2004 Kit for 40 labeling |
|
PCR DIG Probe Synthesis Kit
25 amplification/labeling reactions with a final reaction volume of 50 l each One reaction can produce enough labeled probe to analyze 650 cm2 of blot |
|
In situ hybridization for TiLV
DIG-labeled TiLV probe Probes were prepared using DIG-labeling Mix (Roche, Germany) • Plasmid pGEM-415 bp was used as a template in the labeling |
|
Making Dig labeled RNA Probes
Making Dig labeled RNA Probes 1) Amplify DNA via pcr, using plasmid primers ( ie T7,T3) 2) Put on gloves to protect the RNA you will make from the RNAse on |
|
A Method for High Quality Digoxigenin-Labeled RNA Probes for In
Digoxigenin (DIG)-labeled RNA antisense probes are widely used for in situ hybridization due to their high sensitivity and specificity DIG-labeled RNA probes |
![How do you set label for PDF pages in toolbar? [A-PDFcom]](https://www.biologycorner.com/wp-content/uploads/cell_labeling_for_anatomy_numbered.png "How do you set label for PDF pages in toolbar? [A-PDFcom]")
![PDF] International Labeling Requirements for Medical Devices Medica](https://www.labelsandlabeling.com/sites/labels/lnl/files/styles/680x380_-_news_image/public/news/iso_website.jpg "PDF] International Labeling Requirements for Medical Devices Medica")
