dig rna labeling
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DIG RNA Labeling Kit (SP6/T7)
Transcription of control DNA 1 by T7 RNA polymerase according to the standard protocol produces DIG-labeled “antisense” Neo transcripts of 760 bases in length Use as control in labeling and hybridization reactions Contains DNA fragments of 798 and 3281 bp due to cleavage at the 2 Pvu II sites on the plasmid |
What is DIG-labeled RNA used for?
DIG-labeled RNA is used in a variety of hybridization techniques: The DIG RNA Labeling Mix is especially designed for the use with SP6,T7 and T3 RNA polymerases from Roche which are supplied with an optimized transcription buffer. Function tested in the DIG RNA Labeling Kit and in the DIG Nucleic Acid Detection Kit. For life science research only.
How is dig incorporated into RNA?
DIG is incorporated into the RNA at approximately every 25-30 nucleotides. The DNA template to be transcribed is cloned into the polylinker site of an appropriate transcription vector, which contains promoters for SP6 and or T3 and T7 RNA polymerases.
How is DIG-labeled RNA synthesized?
In the standard reaction about 10 g „full length“ DIG-labeled RNA is synthesized from 1 g linearized plasmid DNA with an insert of approx. 1 kb in 2 h. Larger amounts of DIG-labeled RNA can be obtained by scaling up the reaction components.
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DIG RNA Labeling Kit (SP6/T7)
Use the DIG RNA Labeling Kit (SP6/T7) to label RNA with digoxigenin-UTP by in vitro transcription. DIG-labeled RNA is used for hybridization to: • Northern |
| DIG RNA Labeling Mix |
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HighYield T7 Digoxigenin RNA Labeling Kit
DIG-11-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7. RNA Labeling Polymerase |
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For Nonradioactive In Situ Hybridization
Taq DNA Polymerase) as well as RNA Polymerases (SP6 T3 |
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DIG Northern Starter Kit
Use your PCR product directly for labeling see section |
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The DIG System - Labeling and Detection of Nucleic Acids
DIG labeling produces high sensitivity and low background. Easily label. - DNA. - RNA. - oligonucleotides. Label the probe using familiar efficient procedures. |
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Fluorescein RNA Labeling Mix 10x conc.
RNA labeling with fluorescein-12-UTP by in vitro transcription with SP6 T7 |
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RNA Labeling using In Vitro Transcription
Labeled RNA probes permit very sensitive detection. DIG-labeled RNA probes produce higher sensitivity. (10 to 100-fold) than DIG-labeled DNA probes for |
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DIG RNA Probe Synthesis and Purification Lili Jing* Department of
this protocol is a detailed description of synthesizing an antisense DIG-labeled RNA probe for in situ hybridization using the DIG RNA labeling kit from |
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RNA Labeling using In Vitro Transcription
Labeled RNA probes permit very sensitive detection. DIG-labeled RNA probes produce higher sensitivity. (10 to 100-fold) than DIG-labeled DNA probes for |
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DIG RNA Labeling Mix, 10 × conc - Sigma-Aldrich
DIG-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription DIG-11- UTP is incorporated by SP6, T7 and T3 RNA poly- |
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DIG RNA Labeling Kit (SP6/T7) - Sigma-Aldrich
Transcription of control DNA 1 by T7 RNA polymerase according to the standard pro- tocol produces DIG-labeled “antisense” Neo transcripts of 760 bases in |
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I In situ hybridization with DIG-labeled probes - Roche Life Science
RNA-RNA in situ hybridization using DIG-labeled probes: the effect of high molecular weight polyvinyl alcohol on the alkaline phosphatase indoxyl-nitroblue |
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DIG RNA Probe Synthesis and Purification Lili Jing - Bio-protocol
this protocol is a detailed description of synthesizing an antisense DIG-labeled RNA probe for in situ hybridization using the DIG RNA labeling kit from Roche |
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Making Dig labeled RNA Probes
Making Dig labeled RNA Probes 1) Amplify DNA via pcr, using plasmid primers ( ie T7,T3) 2) Put on gloves to protect the RNA you will make from the RNAse on |
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HighYield T7 Digoxigenin RNA Labeling Kit - Jena Bioscience
DIG-11-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase |
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A Method for High Quality Digoxigenin-Labeled RNA Probes for In
DIG-labeled RNA antisense probes are easily produced using PCR and in vitro transcription, avoiding tedious cloning steps In this note, we describe a method |
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Title A Large-Scale Whole-Mount in situ Hybridization - CORE
A DIG-labeled RNA probe for WISH was synthesized from a PCR-amplified template which contained an RNA promoter All reactions of PCR and subsequent |
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