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  • What is absolute ethanol?

    Absolute or anhydrous alcohol refers to ethanol with a low water content. There are various grades with maximum water contents ranging from 1% to a few parts per million (ppm). If azeotropic distillation is used to remove water, it will contain trace amounts of the material separation agent (e.g. benzene).

  • What temperature is ethanol at?

    Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). ?) Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula CH3CH2OH.

  • What is the chemical formula for ethanol?

    Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula CH3CH2OH. It is an alcohol, with its formula also written as C2H5OH, C2H6O or EtOH, where Et stands for ethyl.

  • What ethanol is used instead of anhydrous ethanol?

    Rectified spirit, an azeotropic composition of 96% ethanol containing 4% water, is used instead of anhydrous ethanol for various purposes. Spirits of wine are about 94% ethanol (188 proof). The impurities are different from those in 95% (190 proof) laboratory ethanol.

3 Ethanol Grades Used in Molecular Biology

Ethanol has a number of uses in microbiology. It is used in the purification and precipitation of biomolecules, in staining and restaining specimens in histology, in dehydrating tissues before embedding, and in disinfection. There are three grades of ethanol commonly used in the lab. bitesizebio.com

Ethanol For Molecular Biology

You should not use denatured ethanol when precipitating nucleic acids, since some of the additives can interfere with downstream applications. This is a bit tricky to predict because the additives vary between producers, but it is safest to avoid denatured ethanol altogether. Non-denatured ethanol at either 95% or 100% can be used, although when wo

Ethanol For Disinfection

Aside from substantial differences in cost, it doesn’t really matter which ethanol grade you use for disinfection, so most labs use a denatured stock. What does matter is the final ethanol concentration. Ethanol is an effective disinfectant at concentrations between 70% and 90%. Although it may seem counter-intuitive, 70% ethanol seems to be the mo

References

Ali, Y, Dolan, MJ, Fendler, EJ, and Larson, EJ (2001), Alcohols in Disinfection, Sterilization, and Preservation, ed. Block, SS, 5thed, Philadelphia: Lea & Febiger. bitesizebio.com

How to make anhydrous ethanol (100% alcohol)

How to make anhydrous ethanol (100% alcohol)

How to Distill Ethanol to 95%

How to Distill Ethanol to 95%

Chemical and Structural Formula for Ethanol (Ethyl alcohol)

Chemical and Structural Formula for Ethanol (Ethyl alcohol)

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