how to calculate dilution factor


  • How do I calculate dilution factor?

    The formula for dilution factor (or DF for short) is as follows: DF = (final volume of cells + stain)/(initial volume of cells). For example, If you mix your sample 1:1 with AO/PI, you'll need to add 20 uL AO/PI to 20 uL cells, for a total of 40 uL. So, DF = ( 40 uL)/(20uL cells) = 2.
  • How do you find the dilution factor from 1 to 10000?

    Final dilution factor (DF) = DF1 * DF2 * DF3, etc. 1:100 * 100 = 1:10,000. Repeated again (the third step) the total dilution would be 1:100 * 10,000 = 1:1,000,000 total dilution.
  • What is 1 to 5 dilution factor?

    Dilution factor of 1:5 would indicate 1 part solute dissolved in 5 parts solvent = 5 parts in total. The final volume is calculated as part volume x total parts.
  • What is the dilution factor if you add 0.1 ml aliquot of a specimen to 9.9 ml of diluent? The dilution factor is equal to the final volume divided by the aliquot volume: 10 mL/0.1 mL = 1:100 dilution.17 oct. 2016
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