multiply by dilution factor


  • How do you multiply by a dilution factor?

    The number of dilutions is equal to the number of times the dilution factor will be multiplied by itself to equal the starting concentration divided by the final concentration. So with a dilution factor of 10, 10 to the X power is equal to the starting concentration divided by the final concentration.
  • How do I calculate dilution factor?

    The formula for dilution factor (or DF for short) is as follows: DF = (final volume of cells + stain)/(initial volume of cells). For example, If you mix your sample 1:1 with AO/PI, you'll need to add 20 uL AO/PI to 20 uL cells, for a total of 40 uL. So, DF = ( 40 uL)/(20uL cells) = 2.
  • What is 2x dilution factor?

    A two-fold dilution reduces the concentration of a solution by a factor of two that is reduces the original concentration by one half. A series of two-fold dilutions is described as two-fold serial dilutions.
  • Using this terminology, a “10X” stock might be diluted by adding 100 mL of the stock to 900 mL to produce a “1X” working solution.
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