standard solution and 5 µL of prepared mixture (mix) were applied as bands Chromatograms were developed to 4/5 of the plate height, then dried in air, treated with the anisaldehyde solution, heated in 105OC, examined in daylight and photographed with a video-scanner HPLC method HPLC analysis was performed on Luna C18(2)
were stained by submersion into aqueous ceric ammonium molybdate solution (CAM) or acidic ethanolic p-anisaldehyde solution (anisaldehyde) followed by brief heating on a hot plate (≈200°C, 10–15 s) Flash chromatography was performed as described by Still et al (1) , employing silica gel (60 Å pore size, 230–400 mesh, Merck KGA; or 60 Å
allowed to evaporate, then the chromatograms were sprayed with anisaldehyde solution, heated at 105°C for 5-10 minutes and examined in daylight within 10 minutes Results and Discussion Colour Analysis Colour variations of homoeopathic mother tinctures are dependent on various factors including the time of harvest,
Reactions were monitored by TLC analysis (EM Science pre-coated silica gel 60 F 254 plates, 250 µm layer thickness) and visualization was accomplished with a 254 nm UV light and by staining with a PMA solution (5 g of phosphomolybdic acid in 100 mL of 95 EtOH), p-anisaldehyde solution (2 5 mL of p-anisaldehyde, 2 mL of AcOH, and 3 5 mL of
volume/volume For TLC analysis, TLC-silica gel 60 F254 plates were purchased from Merck Applied substances were observed using a UV lamp at 254 nm and 365 nm For UV-inactive substances, dyeing reagents, such as 2 4 anisaldehyde solution in ethanol, were used NMR spectra were recorded on
sprayed with 1 ferric chloride solution in 50 methanol [29] Moreover, a solution of Vanillin-sulfuric acid was used to identify steroidal lichen substances [30] Another reagent used in this regard was p-Anisaldehyde solution with 97 sulfuric acid to reveal phenol, terpenes, sugars, and steroidic nature of available compounds
Solution I: 160mg o-tolidine in 30ml glacial acetic acid, filled to 500ml with distilled water, plus 1g KI solution Solution II: saturated solution of o-tolidine in 2 acetic acid/0 85 KI solution (1:1, v/v) Procedure A Place chromatogram 15-20min in a chlorine atmosphere (e g , Potassium permanganate +10 Hydrochloric acid)
To a 0 ºC solution of 3,5-bis(trifluoromethyl)benzoic acid (2 50 g, 9 61 mmol) in DCM (24 mL) and DMF (10 drops) was added oxalyl chloride (1 65 mL, 19 2 mmol) dropwise under an argon atmosphere The solution was stirred at 0 ºC for 15 min then warmed to room temperature for 1 5 hours
25ml with methanol The resulting solution had a concentration of 1mg/ml (solution A) Accurately pipetted out 1 0 and 1 25ml of solution A standards at the same concentration levels on five consecutive in to two boiling tubes, prepared the drug reagent complex as discussed and made up the volume with alcohol The
Reagents as outlined in Chapter 4 (4 1 1) of Ph Eur Reagecon, as a specialist manufacturer of laboratory reagents has now introduced the range of Reagents
EP solutions
CARLO ERBA Reagents products are classified according to their level of purity and suitable for being used in laboratory analysis or industrial preparations
Carlo Erba Catalogue Chemicals
The solution is ideal for applications involving the use of large volumes of u Acetate buffer pH 6 0 > RS-For analysis according to Ph Eur Chap 4 1 3 Code
catchem
Anisaldehyde RE - Pure 100 ml 39 90 PC 123-11-5 611007301 Anisaldehyde solution RS - For analysis according to Ph Eur Chap 4 1 1 100 ml 86 90
Carlo Erba
according to the total alkaloids of the specific drug, so that DAB 10, Ph Eur, I, OAB, Helv, VII, Ill' 88, USP CH 3 Harman Harmine Harmol Reserpine o Serpentine R1 = R2 = H R1 = R2 Anisaldehyde sulphuric acid reagent (AS No 3)
WAGNER+AND+BLADT plant drug analysis a thin layer chromatography atlas