What is cell migration?
Cell migration is an evolutionarily conserved, multifaceted process which allows individual cells and cell groups to move, change position and build, or maintain tissues and organs for embryonic development as well as homeostasis and regeneration (Sonnemann & Bement, 2011;
Does removal of silicone inserts affect cell migration rate?
It has been observed that the removal of silicone inserts can damage the culture surface coating and affect the cell migration rate [ 33 ]. We assume this effect is present in all cell samples so the comparative migration analysis performed by the quantification methods is not affected.
How do we characterize cell migration in the scratch assay?
In this way, we characterize cell migration in the scratch assay by the slopes of a series of linear approximations to the interface position over time in these windows.