Formalin-fixed paraffin-embedded (FFPE) tissue is the most common method of tissue preparation for diagnostic histopa- thology and results in paraffin blocks that are retained for archival purposes
Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining
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16 déc 2016 · Bouin's fixed tissues require more extensive washes However, many research labs store tissues in 70 EtOH at 4o for months In our experience, storage in 70 EtOH is less problematic than either over-fixing in formaldehyde solutions or excessive time in 95-100 EtOH or other organic solvents, such as xylene
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However, formalin fixed tissues showed some peculiarity such as improper fixation, is the superior alternative to formalin for preservation of tissue morphology
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Epitope mask- ing especially affects formalin-fixed tissue stored for lengthy periods of time and this may pose a serious problem when tissue is over-fixed ( e g ,
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28 juil 2005 · from fixed and embedded tissues by using housekeeping genes as reference In contrast, tissue autolysis of up to 24 h and long-term storage
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2 jan 2020 · Obtaining, handling, and storing formalin-fixed, paraffin embedded (FFPE) tissue sections presents unique challenges to global pharmaceutical
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