nucleic acid is composed of six groups so that when it is sub- mitted to hydrolysis with mineral acids it produces the six sub- stances guanine, adenine, cytosine,
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It produces the required amount of guanine, but not a trace of adenine, by hydrolysis with sulfuric acid Its entire phosphoric acid is set free by heating with 5 per
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nucleic acids can be detected in aqueous extracts HC1O4 It will be noted that following acid hydrolysis a small additional yield of DNA and RNA Some
degraded to yield phosphoric acid, pentoses, Nucleic acids are long, unbranched chains of sugar and DNA is more sensitive to acid hydrolysis than RNA
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Heterocyclic Bases—Pyrimidines • DNA and RNA RNA only DNA only Slide 5 Heterocyclic Bases – Enzymatic Hydrolysis of Nucleic Acids • Many different kinds of nucleases in nature • Hydrolysis of Produces 5'-phosphate products
Chapter
nucleic acid (RNA) and the deoxyribose nucleic acid (DNA), which on hydrolysis yield the sugar ribose and deoxyribose respectively Page 4 Nucleic acids
nucleic acid
hydrolysis of nucleic acids liberates nitrogenous bases, a five-carbon sugar, hydrolysis, but purine nucleosides are easily hydrolyzed in acid to yield the free
Nucleotide Chapter
mitted to hydrolysis with mineral acids it produces the six sub- Kossel also showed that while yeast nucleic acid contains groups.
acid also yield levulinic and formic acids. Brown and Johnson. (1) likewise obtained levulinic and formic acids on acid hydrolysis of the nucleic acid of
nucleic acid yields all four of the nuclein bases. While we do not and hypoxanthin are formed in the hydrolysis of thymus nucleic acid by Steudel's ...
It produces the required amount of guanine but not a trace of adenine
sulphuric acid at 150-160° for 5 hours (the usual conditions for the hydrolysis of nucleic acid to yield pyrimidines) and a little of the neutralized
???/???/???? Acid hydrolysis of protein-free mixtures of nucleotides nucleosides
The known nucleic acids are of two types-the ribonucleic acids yielding upon total hydrolysis the sugar D-ribose
At 100 W very low hydrolysis yields (?10.0%)
Whereas the ribose nucleotides on hydrolysis with acids yield strongly reducing mix- tures the thymus nucleic acid shows but the weakest reduction.
???/???/???? depurination and yield DNA–protein cross-links in ... cribed to slow hydrolysis (depurination) in duplex DNA resulting.
RNA is a polynucleotide that upon hydrolysis yields D-ribose phosphoric acid and the four bases adenine guanine cytosine and uracil DNA is a polynucleotide that yields D-2 -deoxyribose phosphoric acid and the four bases adenine guanine cytosine and thymine
Nucleic Acids Two classes DNA (Deoxyribonucleic Acid) RNA (Ribonucleic Acid) Polymers of nucleotides DNA carries genetic informationin the form of nucleotide sequence Central Dogma of Biochemistry DNA ®RNA ®Protein (Figure 11 1) Slide 3 Nucleotides Composition Heterocyclic Base Pentose Phosphate
hydrolysis of the acid and claimed to have separated the four nucleosides from the same nucleic acid gave comparable yields of guanosine (m p and mixed m p 237-240°) and adenosine (m p
Up to the present only methods of enzymatic nature have been available for the specific partial hydrolysis of the nucleic acid chain such as treatment with pancreatic ribonuclease (RNase)
What is the hydrolysis of nucleic acids?
HYDROLYSIS OF NUCLEIC ACIDS 2015-043631 1Department of Biology, College of Science, University of the Philippines Baguio March 14, 2019 ABSTRACT Nucleic acids namely, RNA and DNA both undergo the process of hydrolysis producing products such as phosphates, pentose sugars and heterocyclic bases.
How is the glycosidic bond of DNA and RNA hydrolyzed?
The glycosidic bond of DNA and RNA is hydrolyzed by acids. In acid hydrolysis, the purine acid has been added. This phenomenon occurs both in DNA and RNA. The order of stability where d stands for DNA while r stands for RNA.
Which hydrolysate is more susceptible to hydrolysis than DNA?
For test for purine bases, acid hydrolysates of DNA and RNA exhibited positive result and lastly, for the test for inorganic phosphates, basic hydrolysate of DNA showed negative result. RNA is more susceptible to hydrolysis than DNA, especially in basic hydrolysis due to the presence of 2’ hydroxyl group.
What is the effect of acid on DNA hydrolysis?
1. Effect of Acid: {pH3 and complete hydrolysis (also cleaving of phosphodiester bond) into components at pH
THE PARTIAL ENZYMATIC HYDROLYSIS OF YEAST NUCLEIC ACID