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17 mar 2022 · 9:00 Human genome: from basics to disease (T) Isabel Rivera, FFUL, Portugal 14:30 Basics onto genetic tools: genetic engineering and
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Scientific Tools in Biological Assays
Principles of Cell Cultures and Genetics
Day 1
17 th March
8:45Welcome Alexandra Brito, FFUL, Portugal
9:00Human genome: from basics to disease (T)Isabel Rivera, FFUL, Portugal
9:45 Epigenetics in health and disease (T)Paul Peixoto, UniversitéBourgogne Franche-Comté, France
10:30 Break
11:00 Pharmacogenetics: from principles to biomarkers and personalized medicines(T) Elsa Rodrigues, FFUL, Portugal
11:45 Proteogenomicsand its applications to biology and precision medicine (T) Rahman Jamal, UniversitKebangsaan, Malaysia
12:30 Lunch
13:45Challenges and emerging directions in single-cell analysis(T) Guo-Cheng Yuan, HarvardChan School of Public Health, USA
14:30 Genome editing: tools and principles (T)Manuel Gonçalves, Leiden UniversityMedical Center, TheNetherlands
15:15 High-throughput and high-content screening technologies for RNA-based therapies (T)Miguel Mano, University
of Coimbra, Portugal
16:00 Break
16:30 Why Networks Matter: Embracing Biological Complexity (T) John Quackenbush, Harvard TH Chan School of
Public Health, USA
17:15 Bioinformatic tools in genome analysis(P) Filipa Vale, FFUL, Portugal
18:00 End of session
Faculty
of
Pharmacy
(FF),
University
of
Lisbon
(UL), Portugal March 17 -
19, 2022
Website: https://www.ff.ulisboa.pt/https://www.ff.ulisboa.pt/?lang=en
Day 3
19 th March
9:00Evaluation of genetic modulation efficiency: immunocytochemistry (P; VL) Ana Rita Garcia, FFUL, Portugal
9:45 Evaluation of genetic modulation efficiency: in situ hybridization (P; VL) Joana Godinho-Pereira, FFUL, Portugal
10:30 Break
11:00 Evaluation of genetic modulation safety and efficacy: viability and migration (P; VL) Ana Rita Garcia, FFUL, Portugal
11:45 Genomic assay: single cell and spatial transcriptomics (T) Kyoung Jae Won, Universityof Copenhagen, Denmark.
12:30Course assessment and closingAlexandra Brito, FFUL, Portugal
Organization
Alexandra Brito
FFUL
Joana Pereira
FFUL
Rita Garcia
FFUL Contact: Alexandra Brito (Coordinator) mariabrito@campus.ul.pt
Day 2
18 th
March
9:00MicroRNAs: small but powerful regulators of gene expression (T)Marta Olejniczak, Polish Academy of Sciences,
Poland
9:45MicroRNAs as biomarkers and therapeutic targets(T) Alexandra Brito, FFUL, Portugal
10:30 Break
11:00 MicroRNAs as players in brain pathology (T) Eleonora Aronica, University of Amsterdam, The Netherlands
11:45RNA interference technology to overcome cancer chemoresistance using non-viral vectors for assisting
siRNA uptake by malignant cells (T) AmáliaS Jurado, University of Coimbra, Portugal
12:30 Lunch
13:45 Next generation sequencing analysis of miRNAs (P)Jessy Slota, University of Manitoba, Canada
14:30 Basics onto genetic tools: genetic engineering and recombinant plasmid construction (P) Madalena
Pimentel, FFUL, Portugal
15:15 Bacterial plasmid production: competent bacteria transformation and plasmid DNA purification (P; VL) Inês
Figueira, CEDOC, Portugal
16:00 Break
16:30Basics in cell cultures: human and mouse cell lines (P; VL) Rafaela Silvestre, FFUL, Portugal
17:00Gene modulation: siRNA and plasmid transfection (P; VL) Sara Caetano, FFUL, Portugal
17:30 Molecular biology tools: RNA extraction and quantification, cDNA synthesis and RT-qPCR) (P; VL) Joana
Godinho-Pereira, FPUL, Portugal
18:00End of session
Fully online sessions
Theoretical(T): 9.75 h
Practical(P): 4.5 h
Virtual Lab(VL): 2.25 h
Total: 16.5 h
Officiallanguage: English
Timetable: Western EuropeanTime
Recordedsessions: available1 week
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