[PDF] Kathy Y Wei Stanford Bioe PhD + UW Comp Sci BS + 4 yr post

31065_3KYW_resume.pdf

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Kathy Y. Wei

Scientist - Protein Engineering & Design

kywei [at] alumni [dot] stanford [dot] edu

I N T E R E S T S

Stanford Bioe Ph.D. + UW Comp Sci B.S. + 4 yr post-grad experience interested in computational protein design

E D U C A T I O N

Stanford University Sep 2009 - Sep 2015

o GPA: 4.027/4.000 o Ph.D. in Bioengineering o MS in Bioengineering

University of Washington Sep 2005 - Jun 2009

o GPA: 3.97/4.00 o BS in Bioengineering with Honors, Summa Cum Laude o BS in Computer Science and Engineering, Summa Cum Laude

P U B L I C A T I O N S

o in submission. De novo design of proteins with two isoenergetic but structurally divergent ground states.

o in preparation. Differential affinity and specificity of tandem calponin homology domains. o in submission. Computational design of a de novo homo-trimeric amantadine-binding protein. o in submission. De novo design of tunable pH-driven conformational switches. o 2018 Science - Accurate computational design of multipass transmembrane proteins.

o 2015 J of Biol Eng - Engineering synthetic ligand-responsive RNA devices for controlling the cell cycle.

o 2013 Annu Rev Chem Biomol Eng. - Synthetic biology: advancing the design of diverse genetic systems.

o 2013 Biotechnol Bioeng - A yeast-based rapid prototype platform for gene-control elements in mammalian

cells.

S E L E C T T E C H N I C A L S K I L L S

Computational:

o Computational protein design using Rosetta, PyMol, Foldit, Phenix, Coot, proficient in Matlab, Java,

C++, experience in Python, HTML

Protein:

o Protein expression and purification (bacterial, mammalian), incorporation of non-natural amino-acids,

protein labeling, SEC-MALS, CD, SAXS, x-ray crystallography

Cell culture:

o Cell line culture, non-viral & lentiviral gene delivery, stable cell line creation, flow cytometry, cell cycle

analysis, macrophage bead eating assays

Misc:

o Molecular cloning, qRT-PCR, preparation of reconstituted lipid systems (GUVs, SUVs, SLBs), fluorescence confocal and TIRF microscopy

R E S E A R C H E X P E R I E N C E

Scientist - Protein Engineering & Design, Amgen, South San Francisco CA Mar 2019 - present o Structure-based protein engineering group w/in Protein Technologies o Blend structural analysis, computational protein design, and experimental protein production and characterization

o Fusion protein construct design, complex immunogen design, protein stabilization, affinity modulation

of protein/protein interactions, and/or de novo protein design o Support therapeutic areas (e.g. oncology, inflammation, cardiometabolic, etc.) & early discovery

POSTDOCTORAL

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Prof. Daniel Fletcher, Dept. Bioengineering, University of California Berkeley Jan 2017 - Feb 2019

o Engineer proteins to promote antibody mediated cellular phagocytosis at macrophage-cancer interface

Prof. David Baker, Dept. Biochemistry, University of Washington Oct 2015 - Dec 2016 o De novo design of protein conformational switches o Computational design of multipass transmembrane proteins o Design of ligand-induced oligomerization of symmetrical helical bundle

GRADUATE

Prof. Christina D. Smolke, Dept. Bioengineering, Stanford Jan - Mar 2010; Jun 2010 - Aug 2015

o Engineering synthetic ligand-responsive RNA devices for controlling the cell cycle (Dissertation).

o Translation of ribozyme-based gene expression control devices from yeast to mammalian cells. Prof. Fan Yang, Dept. Bioengineering & Orthopaedic Surgery, Stanford Mar 2010 - Jun 2010 o Engineering adult stem cells as a targeted anti-cancer therapeutic (Rotation). Prof. Markus W. Covert, Dept. Bioengineering, Stanford Sep 2009 - Dec 2009 o Integrating a viral component into an in silico model of E. coli (Rotation).

P R O F E S S I O N A L E X P E R I E N C E

Mentoring of direct reports Ja n 2012 - Mar 2017

o UC Berkeley: 2 graduate students o UW: 1 master student, 1 postdoctoral scholar o Stanford: 2 undergraduates, 5 graduate students Course TA for BioE/E80, Stanford University Apr 2011 - Jun 2011 o Designed and taught MATLAB tutorial o Created and graded problem sets and exam questions Summer Software Development Intern, Amazon.com, Seattle, WA Jun 2008 - Sep 2008 o Selective caching on non-keyword searches - Java, Perl. o Implement new MP3 search pages - Perl, HTML.

SAT Course Instructor, Kaplan Test Prep and Admissions, Seattle, WA May 2006 - Feb 2007

o Taught several courses of 5-15 students in Seattle and Bellevue, WA.

S E L E C T A W A R D S

o Siebel Scholar 2015 o 1 year award to 85 top students from the world's leading graduate schools o NDSEG Fellowship 2009 o 3 year award from the DoD to honor highly competitive graduate students o NSF GRFP 2009 o 3 year award for outstanding graduate students nationwide o University of Washington College of Engineering Dean's Medal for Academic Excellence 2009 o #1 undergraduate of the College of Engineering cohort o University of Washington Outstanding Senior in Computer Science and Engineering 2009 o 1 of top 3 of the undergraduate Computer Science cohort o Barry M. Goldwater Scholar 2008 o 1 of 4 awards per year to all undergraduate researchers at University of Washington

O U T R E A C H a n d L E A R N I N G

Cold Spring Harbor X-Ray Methods in Structural Biology Course Oct 2018 Bad Science Podcast - Bladerunner w/Paul Scheer Sep 2018 Life Sciences Research Weekend, Pacific Science Center Nov 2015 Invited lectures, Stanford University Online High School Jul 2013, Oct 2013, Jul 2014 Biomedical Engineering Society (BMES) Mentor, Stanford University Oct 2011 - Sum 2015 PADI Open Water SCUBA Certification Jun 2008 - present

Foreign Languages

o Chinese - Mandarin and Shanghai dialect