[PDF] Colonial Characteristics




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[PDF] Colonial Characteristics

Colonial Characteristics Introduction Colony morphology is the visual culture characteristics of a bacterial colony on an agar plate Observing colony

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This job aid is a component of the free, on-demand CDC training course “Biochemicals and Gram Positive Organism

Identification." Find the course at https://www.cdc.gov/labtraining.

Colonial Characteristics

Introduction

Colony morphology is the visual culture characteristics of a bacterial colony on an agar plate. Observing colony

morphology is an important skill used in the microbiology laboratory to identify microorganisms. Colonies need to

be well isolated from other colonies to observe the characteristic shape, size, color, surface appearance, and

texture. Another important characteristic of a bacterial colony is hemolysis. Hemolysis is the result of the lysis of

the blood cells that are a component of Blood Agar. Colony Morphology Characteristics:

Size:

General Guidelines:

large (>1mm) medium (approximately =1mm) small (<1mm) pinpoint (<0.5mm)

Surface Appearance:

smooth rough glistening dull wrinkled spreading mucoid

Color:

pigmentation (white, pink, purple, etc.) opaque transparent Shape: Form Hemolysis Margin Hemolysis: Alpha ;ɲͿ Beta ;ɴͿ Gamma ;ɶ
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