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Corneal buttons after routine processing were embedded and cut with a microtome at 5-7 microns, and the serial sections obtained were stained with hematoxylin and eosin stain The periodic acid-schiff (PAS) stain was also performed routinely to assist in assessing histopathology, visualization of the epithelial An insight into corneal button
histology and TEM Corneal buttons were ?xed in Carson From the Departments of 1Ophthalmology and 2Pathology, Hoˆpi- For each corneal button, one half was routinely pro-
3436 cells/mm 2and 1919 cells/mm ; corneal diameters were 11 75 mm and 12 50 mm; IOPs were 20 mm Hg and 15 mm Hg; and cup to disc ratios 0 1 and 0 5 in the right and left eyes, respectively The reduced ECD in the left eye was due to a larger corneal diameter and loss from glaucoma and its surgical intervention 5
On histology, stromal fibrosis was observed in the removed corneal button, and molecular assays revealed increased levels of type 1 and 5 collagens Conclusion: The IOP measurement using the conventional applanation-based tonometry can be inaccurate in congenital corneal opacity which is marked by corneal fibrosis