DNA ligase is an important cellular enzyme, as its function is to repair broken phosphodiester bonds that may occur at random or as a consequence of DNA
? Ligases join nucleic acid molecules together ? Polymerases make copies of molecules ? Modifying enzymes remove or add chemical groups
NPTEL – Bio Technology – Genetic Engineering Applications DNA ligase enzyme is used by cells to join the “okazaki fragments” during DNA
It plays an important role in repairing single-strand breaks in duplex DNA in living organisms, but some forms such as DNA ligase IV) may specifically repair
There are many more functions that proteins serve, e g another important role is as an enzyme Like their functions, protein molecules vary tremendously They
Furthermore, the process requires 'biological glue', i e enzymes called ligases, to join the insert and vector together A generic gene cloning process may