All technical literature is available at: www promega com/protocols/ Visit the web site to verify that you Setting up a Restriction Enzyme Digestion
restriction-enzymes-protocol.pdf
Restriction Enzyme Activity in Promega 10X Buffers, Reaction Temperature and Heat Inactivation The 10X Reaction Buffer supplied with each restriction
restriction-enzyme-activity-in-promega-buffers.pdf
Composifion of Promega Restricfion Enzyme Reacfion Buffers (1X) 1X Restriction Enzyme Buffer Composition pH Tris-HCl MgCl2
restriction%20enzyme%201x%20buffer%20composition.pdf
Restriction Enzyme Activity in Promega 10X Buffers, Reaction Temperature and Heat Inactivation The 10X Reaction Buffer supplied with each restriction
Promega_RE_Buffer_Table.pdf
Sterile, deionized water (MiliQ) 16 µl Restriction enzyme 0 5 µl Restriction Enzyme Buffer 10X 2 µl 2) Add 9 2 µl of the enzyme master mix to a tube 3)
T--UPF_CRG_Barcelona--protocol_enzymespromega.pdf
Therefore, a DNA cleanup step is not required before proceeding to a ligation reaction TSAP is fully active in all Promega restriction enzyme reaction buffers
tsap-thermosensitive-alkaline-phosphatase-protocol.pdf
Restriction Enzymes That Cut the pSP73 Vector Between 1 and 5 Times Page 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll
pSP73_map_MCS_seq.pdf
Promega has state-of-the art facilities for the manufacture of proteins from fermentation all the way through Over 200 modifying and restriction enzymes
custom-manufacturing-custom-enzymes.pdf
Materials: Restriction enzymes and DNA modifying enzymes were purchased from Promega (Madison, WI) and New England Biolabs (Ipswich, MA)
c3cc46517a.pdf