The cell cycle profile of a sample can be determined by staining the DNA membrane-permeable DNA dye to an existing surface staining protocol
Flow_TechNotes_Cell-cycle-analysis_20170918.pdf
The excitation of PI at 488 nm facilitates its use on the benchtop cytomters Protocol for staining whole cells with PI: 1 Harvest cells and prepare single
DNACellCycleAnalysiswithPI.pdf
Commonly used DNA dyes used for flow cytometric cell cycle analysis include propidium iodide (PI), 4',6-Diamidino-2-phenylindole dihydrocloride (DAPI),
Cell%20Cycle%20Tutorial.pdf
CellCycle Analysis The cell cycle profile of a sample can be determined by staining the DNA with a fluorescent dye and PI cell cycle staining protocol:
cell-cycle-analysis-pi.pdf
This is a method for cell cycle analysis using propidium iodide (PI), that is, further optimisation (see “Internal antigens and cell cycle” protocol
facilities-flowcyt-pi_cell_cycle.pdf
For cells that must be stained prior to EtOH fixation, stain the cells according to the manufacturer's protocol 3 Wash cells in staining buffer before
Cell-Cycle%20Analysis%20Using%20the%20BD%20FACSArray%E2%84%A2%20Bioanalyzer.pdf
23 avr 2016 · (DNA / cell cycle staining) distinguished by Flow Cytometry (due to their DNA-content) Protocol for DNA-Analysis/ Cell cycle
Cell-Cycle-Steffen-S.pdf
Cell Cycle Analysis by Propidium Iodide (PI) Staining Adherent cells: 1 trypsinized 2 suspended in medium + 10 FCS 3 centrifuged (1000 rpm, 5 min)
Cell_Cycle_with_PI.pdf
The protocol below may be used for DAPI measurements alone and should not be combined with other dyes or surface markers since alcohol based fixatives degrade
CellCycle_-DAPI_Alcohol.pdf