The cell cycle profile of a sample can be determined by staining the DNA Propidium Iodide (PI) – Blue/Green lasers, also stains RNA
Flow_TechNotes_Cell-cycle-analysis_20170918.pdf
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI) It can be used to stain whole cells or isolated
DNACellCycleAnalysiswithPI.pdf
Image-Based Analysis of Cell Cycle Using PI Introduction: Cell Cycle The process of DNA replication and cell division is known as the cell cycle
image-based-analysis-of-cell-cycle-using-pi.pdf
Special considerations must be taken when optimizing a flow cytometer for cell cycle analysis Because the DNA histogram is the final product of the flow
Cell%20Cycle%20Basics.pdf
Cell Cycle Analysis by Propidium Iodide (PI) Staining Adherent cells: 1 trypsinized 2 suspended in medium + 10 FCS 3 centrifuged (1000 rpm, 5 min)
Cell_Cycle_with_PI.pdf
Propidium iodide (PI) is the most widely used fluorescent dye for staining DNA in whole cells (or isolated nuclei) PI intercalates into the DNA helix of fixed
Cell-Cycle%20Analysis%20Using%20the%20BD%20FACSArray%E2%84%A2%20Bioanalyzer.pdf
These properties favor the use of PI for evaluation of (apoptotic) cell death or for cell cycle analysis When excited by a 488 nm laser, the fluorescence
PIF_F.121-1.pdf
This is a method for cell cycle analysis using propidium iodide (PI), that is, using the fluorescent nucleic acid dye PI to identify the proportion of cells
facilities-flowcyt-pi_cell_cycle.pdf