[PDF] TUBE METHOD Principle Serial dilution is a common technique

How do I perform a serial dilution?

Serial dilutions can be performed in tubes (microfuge or 12 X 75 mm) or in the wells of plates (depending on the volumes you require). It is important to know what volume of the dilution you will need and the range of concentrations that you desire.

How do you calculate serum dilution?

Set up the problem as 5 ml serum + X ml saline = 25 ml of the final solution. X = 25 - 5 X = 20 ml of saline. Microbiology BIOL 275 Dr. Eby Bassiri ebassiri@sas.upenn.edu. The serum dilution is the amount of serum in the amount of total solution; hence, this is a 5/25 serum dilution which would equal a 1/5 dilution.

What is a 10 fold dilution used for?

Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes (for a standard curve for example). Serial dilutions are also commonly used to avoid having to pipette very small volumes (1-10 µl) to make a dilution of a solution.

How do you detect the end point of a microorganism dilution?

To detect the end point, multiple serial dilutions are inoculated into a suitable growth medium, and the development of some recognizable characteristic, such as acid production or turbidity, is used to indicate growth (the presence of at least one viable microorganism in the diluted sample).