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Drosophila

Cinderella

of Genetics

Drosophila

Drosophila

DrosophilaLIFE into

Drosophila

Drosophila

o

Preface

Drosophila

Drosophila

classes.

Subhash C. Lakhotia Hassan A. Ranganath

Editor-in-Chief

Co-Editor

Rearing and handling of Drosophila- A primer for

laboratory experiments Govind R. Chaudhary, Akanksha Pandey, Anjali Singh, Vaishali Yadav, Vasudha Dwivedi, Richa Arya and S. C. Lakhotia.

Illustrated anatomy of

Drosophila melanogaster

Homica Arya and Deepti Trivedi

Familiarization with a few mutant phenotypes of

Drosophila melanogaster

H. A. Ranganath and V. Shakunthala

Analysis of

Drosophila

eye pigments by paper and thin layer chromatography

Girish Chandran and S. R. Ramesh

Genetic crosses with

Drosophila melanogaster

to understand Mendelian inheritance Bimalendu B. Nath, J .K. Roy, S.C. Lakhotia and H.A. Ranganath

To study the pattern of inheritance of white eye

phenotype in

Drosophila

H. A. Ranganath

S. R. Ramesh and K. Ravi Ram

To study the balanced lethal system in

Drosophila melanogaster

H. A. Ranganath

To study inheritance of quantitative traits: Numbers of Sex comb teeth and Sternopleural bristles in

Drosophila melanogaster

H. A. Ranganath

Use of paper microscope (Foldscope) for class room teaching of genetics Kelevikho Neikha, Nukshimenla Jamir, Abuno Thepa, Bendangtula

Walling, Abhik Das and Sarat Chandra Yenisetti.

Drosophila

larval stages Kiran Nilangekar, Nidhi Murmu, Minal Ayachit and Bhupendra

Shravage

Contents

1-31 33-50
51-57
59-66
67-73
75-78
79-87
89-92
93-96

97-102

103-1101.

2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Drosophila melanogaster

larvae Shamprasad Varija Raghu, Sanmitha S Jujare and Rajashekhar K. Patil

Study of

in situ ൵

Drosophila

Brijesh Singh Chauhan and S. Srikrishna

Dissection and mounting of imaginal discs of

Drosophila

B.V. Shyamala

Anatomy of the wild type third instar larval fat body

Indira Paddibhatla

Understanding the anatomy of the wild type third instar larval blood cells (hemolymph and lymph gland)

Indira Paddibhatla

Malpighian tubules of

Drosophila melanogaster as a model to study

systemic infection by detecting Antimicrobial peptide expression

Puja Verma and Madhu G. Tapadia

Demonstration of arms race between Drosophila and its parasitoid wasp and the haplo-diploid mode of sex determination in wasps

Indira Paddibhatla

Drosophila

larvae Sanjay Saini, Madhu G. Tapadia and Naveen Kumar Gautam

Assessing functionality of

Drosophila

nephrocytes using silver nitrate Lavi Rani, Sanjay Saini, Neha Shukla, Madhu G. Tapadia and Naveen

Kumar Gautam

Dissection of larval gonads of

Drosophila

Nidhi Murmu, Kiran Nilangekar, Minal Ayachit and Bhupendra

Shravage

Demonstration of the hormonal control of metamorphosis using

Drosophila

as a model

Leena Thorat and Bimalendu B. Nath

Study of mitotic chromosomes of

Drosophila melanogaster

J. K. Roy and S. C. Lakhotia169-173165-168 157-164153-155149-151141-147137-140131-135127-130121-126115-119111-11312.

13. 14. 15. 16. 17. 18. 19. 20. 21.
22.
23.
Study of polytene chromosomes of Drosophila melanogaster

J. K. Roy and S. C. Lakhotia

Live observation of embryonic development of Drosophila

R. Ramya and B.V. Shyamala

Study of genetic basis of segmentation in

Drosophila melanogaster

using embryonic cuticle patterns

Prachi Yadav, S. C. Lakhotia and Jagat K. Roy

Introduction to the embryonic nervous system in

Drosophila

Anuradha Ratnaparkhi

Visualization of

Drosophila

larval body wall muscles and their innervations

Pooja Rai, Rima Saha and S. C. Lakhotia

P-element mutagenesis and characterization of P-element insertional mutations

J. K. Roy and S. C. Lakhotia

Gene knockouts in

Drosophila

using the CRISPR-Cas9 based genome editing Sushmitha Hegde, Prajna Nayak, Deepti Trivedi and Girish Ratnaparkhi The GAL4-UAS system to monitor gene activity patterns and to induce ectopic expression in

Drosophila melanogaster

Prachi Yadav, S. C. Lakhotia and Jagat K. Roy

GAL4-UAS system for genetic labeling and visualization of

Rajashekhar K. Patil and Shamprasad Varija Raghu

Drosophila

Harshita Bhati and Subhash Rajpurohit

Study of sex-comb variations in

Drosophila

species

Kopal Singhal and Sujata Mohanty

Preparation and study of male external genitalia of Drosophila

B.V. Shyamala

Demonstration of heat shock induced gene activity in

Drosophila

S. C. Lakhotia

175-183

185-191

193-198

199-206

207-212

213-218

219-224

225-232

233--237

239-245

247-250

251-254

255-26124.

25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.

Drosophila

Chitrang Dani and Vasu Sheeba

Heat stress tolerance in Drosophila

Chitrang Dani and Vasu Sheeba

Drosophila

Chitrang Dani and Vasu Sheeba

Testing the ability of

Drosophila

to tolerate desiccation stress

Chitrang Dani and Vasu Sheeba

Oxidative stress in

Drosophila

: Measurement of Trolox equivalent antioxidant capacity

Avinash K. Kudva and Rajashekhar K. Patil

Oxidative stress in

Drosophila

: Measuring activities of superoxide dismutase and catalase antioxidant enzymes

Avinash K. Kudva and Rajashekhar K. Patil

Visualization of nucleolus in the larval salivary gland polytene nuclei in

Drosophila melanogaster

using light microscopy

Bimalendu B. Nath and Leena Thorat

Drosophila tissues

Sonia Narwal, Shreyas Iyer and Meghana Tare

Localization of DNA sequence on polytene chromosomes of

Drosophila melanogaster

by in situ hybridization

J. K. Roy and S. C. Lakhotia

RNA in situ hybridization (RISH) to visualize the cellular distribution and abundance of mRNA

Anand K. Singh and S. C. Lakhotia

Spatial localization of a protein by immunostaining

S.C. Lakhotia and J. K. Roy

Comet assay for visualization of DNA damage in haemocytes.

Prem Rajak and Sumedha Roy

Genomic DNA isolation from Drosophila

Manish Pandey, Sakshi Bansal and Geetanjali Chawla

263-266

267-270

271-274

275-278

279-282

283-287

289-291

293-296

297-305

307-314

315-321

323-328

329-33537.

38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.

50. Extraction of genomic DNA from single Drosophila

Limamanen Phom, Mohamad Ayajuddin, Bovito Achumi, Imusosong Longkumer, Zevelou Koza, Priyanka Modi and Sarat Chandra Yenisetti

51. Total RNA isolation from Drosophila using TRIzol based reagent

Manish Pandey and Geetanjali Chawla

Quantitative real time PCR (qRT-PCR) for gene expression analysis Abhik Das, Rahul Chaurasia, Limamanen Phom, Priyanka Modi and

Sarat Chandra Yenisetti

Demonstration of genetic diversity by protein and isozyme analysis

S. R. Ramesh and K. Ravi Ram

Detection of proteins in wing imaginal discs of Drosophila larvae by western blotting using damage-induced p-Jun as an example.

Chaitali Khan and Basuthkar J. Rao

Priyanka Modi, AbunoThepa, Nukshimenla Jamir, Abhik Das and Sarat

Chandra Yenisetti

56. Analysis of male fertility in Drosophila

Tushna Kapoor and Krishanu Ray

Fecundity assay as a readout for xenobiotic toxicity and for genetic

Amartya Mukherjee and Upendra Nongthomba

Drosophila melanogaster

Megha Screening of novel potential anti-cancer agents using Drosophila tumour models

Sushree Sulava and Debasmita Pankaj Alone

60. Visualizing neurons in Drosophila - classical staining techniques

Rajashekhar K.Patil and Shamprasad Varija Raghu

337-340

341-350

351-359

361-371

373-379

381-388

389-394

395-399

401-407

409-418

419-423 52.

53.
54.
55.
57.
58.
59.
A study of the distribution of Campaniform sensilla on the wings of

Drosophila melanogaster

Shamprasad Varija Raghu, Avinash K. Kudva and Rajashekhar K. Patil the appendages of

Drosophila melanogaster

Rajashekhar K. Patil and Shamprasad Varija Raghu

Drosophila

brain using HPLC Mohamad Ayajuddin, Abhik Das, LimamanenPhom, Zevelou Koza,

Rahul Chaurasia and Sarat Chandra Yenisetti

Observing surface topography of Drosophila eye by Scanning

Electron Microscopy

Sonia Narwal, Shreyas Iyer, Arushi Rai and Meghana Tare Nail polish imprint as readout for ommatidial arrays of adult eyes of

Drosophila

Richa Arya, S. C. Lakhotia and Akanksha Verma

Do female

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