LOI N? 92 - 020 / PORTANT CODE DU TRAVAIL EN REPUBLIQUE
LOI N° 92 - 020 / PORTANT CODE DU TRAVAIL EN REPUBLIQUE DU MALI. L'Assemblée Nationale a délibéré travailleurs en service à la date de leur publication.
warrantlist.pdf
RUN DATE: 07/14/22 ACWIL PAUL/PHILI 10/19/1974 09-20114 DWLS/R/D 7/18/2017 DSP GLTY P ... ADAMS BRIAN/MICH 11/18/1996 1901964 BAC .17 MO 11/27/2019.
2020 International Compilation of Human Research Standards
The year of the document's most recent version (or date of initial approval Subpart D: Additional Protections for Children Involved as Subjects in ...
International Compilation of Human Research Standards 2017 Edition
The year of the document's most recent version (or date of initial approval Subpart D: Additional Protections for Children Involved as Subjects in ...
Untitled
OAK RIDGE MUNICIPAL PLANNING COMMISSION. AUGUST 2017. AGENDA. A. Rezoning Requests Date: Property Owner: Location: Zoning: 8/10/2017.
WORLD MIGRATION REPORT 2020
The second workshop of the International Dialogue on Migration 2017 countries such as the Niger and Mali toward Ghana and Côte d'Ivoire.46 A large ...
C:UsersDIOPDocumentsJO 2017
11 Août 2017. JOURNAL OFFICIEL. DE LA. REPUBLIQUE DU MALI. TARIFS DES ABONNEMENTS à compter de la date de réception de la requête pour notifier.
Côte dIvoire: Poverty Reduction Strategy Paper; IMF Country Report
recent data on the population for development planning purposes is the East by Ghana in the North by Burkina Faso and Mali
A New Sequence Type of Neisseria meningitidis Serogroup C
Clifton Road NE MS D-11
Warning: This report is out-of-date. In particular entire time-series of
Completed in 2017–2019 (n=11). Underway/planned (n=5). Not planned. Not applicable. Status a. In addition to the five countries planning a first assessment
Diseases, August
26-29, 2018, Atlanta, GA.
The Journal of Infectious Diseases
2019;220(S4):S190-7
Published by Oxford University Press for the Infectious Diseases Society of America 2019. This work is written by (a) US Government employee(s) and is in the public domain in the US. This Open Access article contains public sector information licensed und er the Open Government Licence v2.0 (http://www.nationalarchives.gov.uk/doc/open-government-licence/version/2/).DOI: 10.1093/infdis/jiz272
A New Sequence Type of
Neisseria meningitidis
Serogroup
C Associated With a 2016 Meningitis Outbreak in MaliYibayiri Osee Sanogo,
1 aIbréhima Guindo,
2 aSeydou Diarra,
2Adam C. Retchless,
1Mahamadou Abdou,
2Souleymane Coulibaly,
2Mahamadou Farka Maiga,
3Mama Coumaré,
3Bakary Diarra,
4Alexander Chen,
1How-Yi Chang,
1Jeni T. Vuong,
1Anna M. Acosta,
1Samba Sow,
4 5Ryan T. Novak,
1 and Xin Wang 1 1 Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Nati onal Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention,Atlanta, Georgia;
2 Institut National de Recherche en Santé Publique, Bamako, Mali; 3Direction Nationale de la Santé, Bamako, Mali;
4Ministère de la Santé, Mali;
5Centre National d'Appui et de
Lutte contre les Maladies/Centre des Vaccins en Dévelopement, MaliIn 2016, Mali reported a bacterial meningitis outbreak consisting of 39 suspected cases between epidemiologic weeks 9 and 17
with 15% case fatality ratio in the health district of Ouéléssebougou, 80 kilometers from the capital Bamako. Cerebrospinal ?uid
specimens from 29 cases were tested by culture and real-time polymerase chain reaction; 22 (76%) were positive for bacterial men
ingitis pathogens, 16 (73%) of which wereNeisseria meningitidis
(Nm). Of the Nm-positive specimens, 14 (88%) wereN meningitidis
serogroup C (NmC), 1 was NmW, and 1 was nongroupable. Eight NmC isolates recovered by culture from the outbreak were
characterized using whole genome sequencing. Genomics analysis revealed that all 8 isolates belonged to a new sequence type (ST)
12446 of clonal complex 10217 that formed a distinct clade genetically similar to ST-10217, a NmC strain that recently caused large
epidemics of meningitis in Niger and Nigeria. ?e emergence of a new ST of NmC associated with an outbreak in the African men
ingitis belt further highlights the need for continued molecular surveillance in the region. Keywords. African meningitis belt; Neisseria meningitidis; outbreak; serogroup C; sequence type.Neisseria meningitidis
is a Gram-negative diplococcal bacterium that can cause life-threatening invasive meningococcal disease (IMD). Invasive meningococcal disease represents a serious public health threat worldwide, and it can present as meningitis, bacteremia, or bacteremic pneumonia. The highest burden of meningococcal meningitis is in the African meningitis belt, a region spanning from Senegal to Ethiopia that experiences seasonal meningitis outbreaks punctuated by periodic epidemics every 5-12 years [1, 2].
Neisseria meningitidis
is classi?ed into 12 serogroups based on the structure of its polysaccharide capsule [ 3 ]. Six serogroups (A, B, C, W, Y, and X) cause the vast majority of IMD cases. Before the introduction of the meningococcal serogroup A con jugate vaccine ([MACV] MenAfriVac) in 2010, most menin gitis cases in the African meningitis belt were caused by N meningitidis serogroup A (NmA). Mass vaccination by MACV resulted in a signi?cant reduction of NmA cases and elimina tion of NmA epidemics in the region [ 4]. Despite this success, non-NmA serogroups, including NmW, NmX, and NmC, re-main a threat in the region and continue to cause periodic
outbreaks and epidemics [ 5-9 ]. It is worth noting that NmC has emerged as an increasing risk for countries of the African continent both within [ 10 ] and outside the belt [ 11 Historically, cases of meningitis caused by NmC have been in frequently reported in the African meningitis belt. ?e ?rst ep idemic associated with NmC was reported in 1975 in northern Nigeria, where 53% of all suspected cases were attributed to NmC [ 12 ]. Localized outbreaks of meningitis caused by NmC have been reported in Burkina Faso in 1979 [ 13 ], in Mali be tween 1988 and 1992 [ 14 ], and in Nigeria in 2013-2014 [ 15 , 16].Neisseria meningitidis
serogroup C emerged as a major public health threat when it caused a large epidemic in Niger in 2015 with 9367 suspected cases and 549 deaths [ 17 ]. ?e largest men ingitis epidemic primarily caused by NmC (83%) was reported in the northern region of Nigeria in 2017 with 14 518 reported suspected cases and 1166 deaths [ 18 ]. Molecular typing re vealed that these recent epidemics were caused by a new NmC strain, sequence type (ST)-10217 belonging to clonal complex (CC) 10217 [ 17 , 19]. Comparative genomics analysis indicated that NmC ST-10217 emerged from an asymptomatically and nasopharyngeally carried meningococcal strain, through the ac quisition of virulence genes such as the NmC capsule genes and a meningococcal disease-associated prophage (MDAΦ) [ 20 Mali, a country in the northwestern part of the meningitis belt, has experienced 17 major epidemics of meningococcal meningitis since 1940, when the disease was first reported in applyparastyle "g//caption/p[1]" parastyle "FigCapt"220Downloaded from https://academic.oup.com/jid/article/220/Supplement_4/S190/5610768 by guest on 25 October 2023
Outbreak of Neisseria meningitidis Serogroup C ST-12446 in Mali • jid 2019:220 (Suppl 4) • S191
the country [1, 21]. The largest epidemics in Mali occurred
between 1969 and 1971, with 18 288 suspected cases and1191 deaths [
22], and in 1997, with 11 228 suspected cases and 1126 deaths [ 21
, 23]. The last outbreak reported in Mali occured in 2008 in Ouéléssebougou, a health district located approximately 80 kilometers south-east of the cap ital Bamako. Fifty-one suspected cases were reported, with a case fatality ratio (CFR) of 12.7%, and the outbreak was due primarily to NmA. However in 2015, 7 NmC cases were reported from the region of Gao, and in 2016, Mali reported a localized outbreak of meningococcal meningitis prima rily due to NmC in the health district of Ouéléssebougou. We describe the 2016 outbreak and characterize the avail able isolates using whole genome sequencing and molecular typing. These isolates were also compared with other strains circulating in the African region.
MATERIALS AND METHODS
The Health District of Ouéléssebougou
The health district of Ouéléssebougou (
Figure 1
) covers an area of approximately 1118 square kilometers with a population of215 775 inhabitants. Ouéléssebougou is one of the 10 initial
health districts in Mali supported by the MenAfriNet project, an international consortium funded by the Bill & Melinda Gates Foundation with the purpose of reinforcing case-based meningitis surveillance systems in 5 key countries in the African meningitis belt and providing a platform to monitor the impact of MACV [24].
Data and Specimen Collection
Epidemiological data, including patient's residence, age, and gender as well as date of disease onset, symptoms, and vac cination status for each suspected meningitis case was col lected on standardized case report forms at district levels. Cerebrospinal fluid (CSF) specimens were collected for each reported suspected case [ 25]. Ethics approval and participant consent was not necessary because specimens were collected for purposes of disease surveillance by Mali Ministry of Health and were deidentified before shipping to the Centers for Disease Control and Prevention ([CDC] Atlanta, GA). As part of the outbreak response, the first 6 CSF specimens collected during the investigation were transported to Bamako and analyzed by Pastorex and cytology at the Center for Vaccine Development laboratory of the National Hospital at Gabriel Touré and subsequently confirmed by culture and real-time polymerase chain reaction (rt-PCR) at the National Reference Laboratory, Institut National de Recherche en Santé Publique (INRSP) in Bamako. The other specimens were analyzed at INRSP using Gram staining and cytology and confirmed by culture and rt-PCR. Whole genome sequencing and Sanger sequencing were performed at the World Health Organization (WHO) Collaborating Center at the CDC in Atlanta. To 25
20
16 cases
4 cases
1 case
Mauritania
SenegalCity of
GaoCity of
AnsongoGaoRegion
Burkina Faso
Bougouni District
Ouéléssebougou
DistrictCity of
Bamako
GuineaNiger
City of
FanaAlgeria
Type ND ST -10217ST-12446
15 10 -15-10-5 Long Lat 05Figure 1.
Distribution of Neisseria meningitidis serogroup C strains in Mali during 2015-2017. Sequence type (ST)-1
0217 (in green) was detected in the Gao region, and
ST-12446 was detected in the Ouéléssebougou district. The STs that were not determined ([ND] in red) were detected in the Gao regi
on.Downloaded from https://academic.oup.com/jid/article/220/Supplement_4/S190/5610768 by guest on 25 October 2023
S192 • jid 2019:220 (Suppl 4) • Sanogo et al identify additional NmC cases in Mali, we reviewed and analyzed national laboratory data from 2015 to 2017. Molecular Characterization by Whole Genome Sequencing and SangerSequencing
For whole genome sequencing, deoxyribonucleic acid (DNA) was extracted from isolates using QIAGEN Gentra Puregene kit (QIAGEN, Hilden, Germany) and sheared to 600 base pairs (bp) using Covaris ultrasonicator (Covaris Inc., Woburn, MA). Dra? genome sequences were generated from 250-bp, paired- end read data generated by an Illumina HiSeq 2500 (CDC Biotechnology Core Facility) as previously described [ 26Genome assemblies of the 8 isolates from Ouéléssebougou were published on PubMLST [ 27
] with identi?ers
58393-58397,
58399, and 58400.
Genes encoding outer membrane proteins and vaccine antigens and multilocus sequence typing (MLST) genes were sequenced and identi?ed using the PubMLST GenomeComparator tool [
27]. Porin A (PorA), Porin B (PorB), and Ferric enterobactin transport (FetA) types are de?ned by their respective variable regions [ 28
]. Molecular pro?le of an iso late was de?ned as serogroup: PorA:FetA:ST(CC) in this study. Neisserial adhesin A (NadA), neisserial heparin binding antigen (NhbA), and factor H binding protein (FHbp) were identi?ed as described in PubMLST [ 29
Phylogenetic Analysis
To understand how the Mali outbreak strains are related to other strains in Mali and in the African continent, a phylogeny was constructed using sequences of the 8 Ouéléssebougou isolates along with sequences of 1 NmC isolate from Gao, 76 isolates from Niger 2015 [ 19 ], 73 isolates from Nigeria 2013-2017 [
20 ], and 1 ST-9367 carriage isolate from Burkina Faso2012 (BL16188) [
20 ]. The genome assemblies were aligned to the BL16188 genome using Snippy v3.1 [ 30]. Columns with gaps or ambiguous bases were masked, retaining a 1 863 980- bp core alignment (87% of BL16188) with 8403 polymorphic sites. Gubbins v1.4.1 (m = 5, i = 10, BL16188) [ 31
] was used to mask recombination events, and a final tree was produced using RAxML v8.2.9 with Stamatakis ascertainment bias correction and 600 bootstraps terminated by MRE bootstopping [ 32
quotesdbs_dbs50.pdfusesText_50
[PDF] date du concours crem 2017
[PDF] date du probatoire 2017 au cameroun
[PDF] date elections parents d'élèves 2017
[PDF] date examen bts 2017 cote d'ivoire
[PDF] date examen dcg 2017
[PDF] date examen dcg 2018
[PDF] date formation des alpes
[PDF] date inscription bts candidat libre 2018
[PDF] date inscription cap petite enfance 2018
[PDF] date inscription cap petite enfance candidat libre 2018
[PDF] date inscription lycée 2017
[PDF] date limite campus france
[PDF] date limite depot de dossier campus france 2017
[PDF] date limite depot dossier pduc 2017