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COULTER LS SERIES

Product Manual

PN 4237214EA (October 2011)

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, California 92821

READ PRODUCT MANUAL AND CONSULT BECKMAN COULTER TRAINED PERSONNEL BEFORE

ATTEMPTING TO OPERATE INSTRUMENT.

HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:

WARNING - Might cause injury.

CAUTION - Might cause damage to the instrument.

IMPORTANT - Might cause misleading results.

CAUTION

The computer installed with your instrument is an integral part of the system. It may not be used to operate

personal software which has not been authorized for use by Beckman Coulter. The computer may only be used

with software that is authorized by Beckman Coulter. Introduction of nonauthorized software may compromise

system integrity and cause operational failures.

CAUTION

Use of equipment in a manner other than specified, might compromise system integrity and cause operational

failures.

Beckman Coulter urges its customers to comply with all national health and safety standards such as the use of

barrier protection. This may include, but it is not limited to, protective eye wear, gloves, and suitable laboratory attire

when operating or maintaining this or any other automated laboratory analyzer.

PN 4237214EA (October 2011) i

REVISION STATUS

Issue A, 5/94

Software version 2.01.

Issue EA, 10/2011

Updated trademark information

Updated all instances of Coulter Corporation or Coulter Electronics to

Beckman Coulter, Inc.

Revised the address to the Beckman Coulter address Updated all illustrations that display the outdated address or Miami address

Deleted the Coulter Care Symbol (CC)

Added the Beckman Coulter, Inc. Customer End User License Agreement

REVISION STATUS

ii PN 4237214EA (October 2011)

PN 4237214EA (October 2011) iii

INTRODUCTION

This manual for the COULTER LS Series is divided into four sections and three appendices: ‡7OH ILUVP VHYHQ ŃOMSPHUV ŃRPSRVH POH 5HIHUHQŃH VHŃPLRQB KRX VORXOG UHMG POHVH chapters before attempting to operate your LS system. ‡7OH VHŃRQG VHŃPLRQ GMLO\ 2SHUMPLQJ 3URŃHGXUHV ŃRQVLVPV RI ŃOMSPHUV 8 through 11. These chapters contain the procedures for obtaining and analyzing data. Chapter 9, Sample Analysis, is divided into four parts, one for each of the sample modules available for LS Series systems. Each part is completely self-contained, so you need only refer to the part for the sample module you are using. Chapters 8 and 11 identify differences between modules where necessary. ‡7OH POLUG VHŃPLRQ $GGLPLRQMO 2SHUMPLQJ HQVPUXŃPLRQV ŃOMSPHUV 12 POURXJO 14 will help you to make the best use of your LS Series instrument. This section explains everything from setting the appearance of graphic and numeric output to making optical models (optical models are necessary to accurately analyze samples containing particles smaller than 10 microns). ‡7OH IRXUPO VHŃPLRQ 6SHŃLMO 3URŃedures and Troubleshooting (chapters 15 through 17), covers maintenance, cleaning, replacement/adjustments and troubleshooting. ‡)LQMOO\ POHUH MUH POUHH MSSHQGLŃHV MP POH HQG RI POH PMQXMOB HI \RXU MSSOLŃMPLRQ is QA/QC, do not overlook Appendix A. Appendix A covers Standard Files, which provide a way to compare results to a standard, and include highlighting of data which falls outside of statistical limits. If you need to correlate data to an existing database of sieve results, or are continuing to compare data to sieve results, see Appendix C, Defining Mesh Weightings (you will first need to read Define Mesh Sizes in chapter 14). Keep this manual within reach as you use your LS system. If you have a problem that you cannot resolve by consulting the manual, contact your local Beckman

Coulter representative.

INTRODUCTION

iv PN 4237214EA (October 2011)

PN 4237214EA (October 2011) v

CONTENTS

1 USE AND FUNCTION, 1-1

1.1 SYSTEM DESCRIPTION, 1-1

1.2 SYSTEM FUNCTION, 1-2

Optical Module, 1-2

Fluid Module, 1-3

Hazardous Fluids Module, 1-4

Micro Volume Module, 1-5

Dry Powder Module, 1-6

Sample System, 1-7

Fluid Module and Hazardous Fluids Module, 1-8

Micro Volume Module, 1-8

Dry Powder Module, 1-8

Computer, 1-8

Printer (Optional), 1-9

Reagent System, 1- 10

Suspension Fluid for the Fluid Module, 1-10

Suspension Fluid for the Hazardous Fluids Module and Micro Volume Module, 1-11

Calibration, 1-12

Control Samples, 1-12

Cleaning Fluids, 1-12

Material Safety Data Sheets (MSDS), 1-12

2 CONTROLS AND INDICATORS, 2-1

2.1 OPTICAL MODULE, 2-1

2.2 FLUID MODULE, 2-3

Hardware Controls, 2-3

Software Controls, 2-3

Fill the Sample Vessel, 2-3

Rinse the Sample Vessel, 2-4

Turn the Pump On or Off, 2-4

Turn the Sonicator On or Off, 2-4

Open or Close the Inlet Valve, 2-4

Open or Close the Drain Valve, 2-5

Adjust Pump Speed, 2-5

2.3 HAZARDOUS FLUIDS MODULE, 2-6

Hardware Controls, 2-6

Software Controls - Computer Monitor Menus, 2-6

Fill the Sample Vessel, 2-7

Filter the Fluid in the Sample System, 2-7

Rinse the Sample Vessel, 2-7

Drain the Sample System, 2-8

Check the Fluid Level in the Sample Vessel, 2-8

CONTENTS

vi PN 4237214EA (October 2011) Check the Calibration of the Level Sensors in the Sample

Vessel, 2-9

Turn the Pump On or Off, 2-9

Turn the Sonicator On and Off, 2-10

Adjust Pump Speed, 2-10

Adjust Sonicator Power, 2-11

Software Controls - Control Panel Menus, 2-11

Filter the Fluid in the Sample System, 2-11

Rinse the Sample Vessel, 2-12

Turn the Sonicator On and Adjust Power, 2-12

Turn the Pump On and Adjust Speed, 2-12

Drain the Sample System, 2-12

Fill the Sample Vessel, 2-12

2.4 MICRO VOLUME MODULE, 2-13

Hardware Controls, 2-13

Software Controls, 2-13

2.5 DRY POWDER MODULE, 2-13

Hardware Controls, 2-13

Software Controls, 2-14

Turn the Vacuum On or Off, 2-15

Turn the Auger/Vibrator (Feed) On or Off, 2-15

2.6 COMPUTER AND MONITOR, 2-16

2.7 KEYBOARD, 2-16

2.8 MOUSE, 2-17

2.9 LS SOFTWARE PROGRAM, 2-17

General, 2-17

MS-DOS® Operating System, 2-18

Microsoft® Windows• Operating Environment, 2-18

Window Components, 2-19

Dialog Box Components, 2-20

Control Menu Commands, 2-21

Main Menu Bar, 2-22

Run Menu Bar, 2-23

Active Status, 2-23

Description, 2-23

Change Active Status, 2-24

Inactive Status, 2-24

2.10 SOFTWARE TECHNIQUES, 2-25

Mouse and Keyboard Techniques, 2-25

Access a Pull-Down Menu, 2-25

Cancel a Menu, 2-25

Select a Command, 2-25

Work with a Dialog Box, 2-26

Select an Option Button and Check Box, 2-26

CONTENTS

PN 4237214EA (October 2011) vii Use a Scroll Bar, 2-27

Select an Item in a List Box, 2-27

Enter Data in an Entry Box, 2-28

Delete Characters in an Entry Box, 2-28

Select a Command Button, 2-28

Work with a Window or Icon, 2-29

Enlarge a Window, 2-29

Restore a Window to Normal Size, 2-29

Move a Window or Icon, 2-29

Change a Window's Size, 2-30

Shrink the LS program to the LS Icon, 2-30

Restore an Application from its Icon, 2-31

Print the Screen Display, 2-31

Print the Main Window, 2-32

Print the Run Window, 2-32

Work with Graph Cursors, 2-32

Add a Single Cursor, 2-32

Add a Second Cursor, 2-33

Delete Cursors, 2-33

Enlarge Part of the Graph Area, 2-34

2.11 MACROS, 2-34

Template, 2-35

Load the Macros, 2-35

Functions, 2-36

3 INSTALLATION, 3-1

3.1 GENERAL, 3-1

3.2 SPECIAL REQUIREMENTS: HARDWARE, 3-1

Environment, 3-1

Electrical, 3-2

Suspension Fluid for Fluid Module, 3-2

Drain for Fluid Module, 3-2

Vacuum for Dry Powder Module, 3-2

3.3 SPECIAL REQUIREMENTS: COMPUTER SYSTEM, 3-3

3.4 SPECIAL REQUIREMENTS: SOFTWARE, 3-3

Define the Connections, 3-3

Change the Setup Directory, 3-4

Install the Printer, 3-5

Define the Printer Features, 3-5

Change Printer Driver, 3-7

Run a Background Test, 3-7

3.5 INTERUNIT CONNECTIONS, 3-7

3.6 FUSES, 3-13

CONTENTS

viii PN 4237214EA (October 2011)

4 OPERATION PRINCIPLES, 4-1

4.1 OVERVIEW OF SAMPLE CYCLE, 4-1

4.2 DIFFRACTION SIZING, 4-1

Components, 4-1

Description, 4-2

Measuring Moving Particles, 4-2

Summary, 4-4

Determining the Particle Size Distribution, 4-4

4.3 PIDS SIZING, 4-4

Components, 4-4

Functions, 4-4

Description, 4-5

4.4 MAKING THE MEASUREMENT, 4-7

5 CALIBRATION, 5-1

6 SPECIFICATIONS/CHARACTERISTICS, 6-1

6.1 PHYSICAL SPECIFICATIONS, 6-1

Power, 6-1 Temperature (ambient operating), 6-1

Humidity, 6-1

Dimensions, 6-1

Altitude, 6-2

Laser, 6-2

Classification, 6-2

Laser Power, 6-2

Wavelength, 6-2

Life Expectancy, 6-2

PIDS Lamp, 6-2

Type, 6-2

Ratings, 6-2

Output, 6-2

Life Expectancy, 6-3

Particle Sizing, 6-3

Range, 6-3

Size Channels, 6-3

Repeatability, 6-3

Run Time, 6-3

Cycle Time, 6-3

Sample Requirement, 6-4

Fluid Module, 6-4

Materials Wetted by Suspension Fluid, 6-4

Capacity, 6-4

Pump Flow Rate, 6-4

Suspension Fluid, Water Supply, 6-4

Drain, 6-4

CONTENTS

PN 4237214EA (October 2011) ix

Hazardous Fluids Module, 6-5

Materials Wetted by Suspension Fluid, 6-5

Capacity, 6-5

Suspension Fluid Consumption, 6-5

Flow Rate, 6-5

Air Source, 6-5

Micro Volume Module, 6-5

Materials Wetted by Suspension Fluid, 6-5

Capacity, 6-5

Dry Powder Module, 6-6

Vacuum, 6-6

Flow Rate, 6-6

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