[PDF] PREPARING SOLUTIONS AND MAKING DILUTIONS





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Dr. Gs DILUTION PRIMER

Diluting a sample by half is a 1:2 dilution. Can we be sure of this? Let's test it out. 200mls divided by 2 (the dilution factor) is 100mls



Laboratory Math II: Solutions and Dilutions

So with a dilution factor of 10 10 to the X power is equal to the starting concentration divided by the final concentration. In this case we will use 1 molar 



DILUTION CHART.pdf

500ml 200ml 100ml 50ml.



PREPARING SOLUTIONS AND MAKING DILUTIONS

material to be diluted) + 4 unit volumes of the solvent medium (hence 1 + 4 = 5 = dilution factor). Example 1: To dilute a streptavidin solution 1:300.



1. dilutions and concentrations. liquid and solid samples

100 ml of a water sample were filtered and then the filter was suspended in 10 ml of saline solution and shaked vigorously. What is the concentration factor?



Dilutions Occasionally a solution is too concentrated to be used as it

dilution factor and so on until the final concentration is known. Example: A 5M solution of HCl is diluted 1/5. The resulting solution is diluted 1/10.



Microbiologics Dilutions Guide

A log dilution is a tenfold dilution meaning the concentration is decreased by a multiple of ten. To complete a tenfold dilution



Answers to Simple Dilution Problems 1) a. 1/10 b. 1/100 c. 1/4 d. 1

The dilution factors are: 2/5 10/1000 and 50/1000. The last one is not actually a dilution factor but is a conversion factor because we take 50 microliter but 



Calculating Nucleic Acid or Protein Concentration Using the GloMax

07-Aug-2009 pathlength of the measurement and an extinction coefficient [1]. ... or RNA constant from Table 1 c. Multiply by the sample dilution factor.



Technical Note 167_Proper Use Of Dilution Fittings On Pumped

If used for VOCs replace the Tygon portions with Teflon as much as possible

NOTE:..

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How to Make Simple Solutions and Dilutions

Unit Definitions

milligram " gram " kilogram " mole "(%)'!#% molarity " molar "" eg)* millimole "#$#%%% millimolar "" eg)% 1. Simple Dilution (Dilution Factor Method).. simple dilution unit volume + solvent + ,dilution factor 0#*0 12 #12"*" )33 4 050
667
)%5 8 050
!).#5

667)%5667

)%5667#3

2. Mixing parts or volumes..

0# 0! 9 7 !.4 8 7 :4 0000 #4

3. Serial Dilution..

serial dilution /etc , 4 total dilution factor product -;- ";-#=;-)=;-'etc !.4 >4??@9)%# three step #.#%% ##%A 33

33%A "#.#%%4!

of the 1:100 dilution 33
#.#%%=#%%"#.#%%%%B less

4. Making fixed volumes of specific concentrations from liquid reagents: V1C1=V2C2..

CD eg

V = volumeC = concentration;

V1C1=V2C2

!#.6 $"C1 )%%A"C2 $"V2 9 V1 )%%A V1 " D

This is your unknown.

C1 "#%%

V2 "")%%A"%)

C2 "")*

C #"C)!7) $7# C "%%*"*%A 6*%A #*%A A)*

5. Molar solutions (unit = M = moles/L)..

6 molarity +#formula weight -E molecular weight !#.To prepare a liter of a simple molar solution from a dry reagent: formula weight E

7-E"#32'

$F%#* #32' $")3#2* !).To prepare a specific volume of a specific molar solution from a dry reagent: -E#G% 8 H $ =-E H $ =-E H "%%)*=%#*$=#G% H "%(I*

6)*%#*

%(I*

6. Percent Solutions (= parts per hundred)..

percent concentrations. E dry mass (g) per volume H $#%%"#%J<7 <7 #%J$<7 !#.4'J$<7 <7#%% 8 #J$ !).,'*%#)J$<7 !'*"2) 62)
<7'*% !'.#*%%*J$ %%*=#*$#%% "%%%I* I*

6%%%I*

E liquid reagents volume per volumeieH !2.4I%J$!I%#%%J !*.,3#))#*J$,5&#%% )#*=3#)$#%% "#3(#

6!#3(#,5&#%%I#*3)#*J$

7. Conversions from % to molarity and from molarity to %..

To convert from % solution to molarity

Molarity = (% solution) * 10

Xxxxx FW

!(.,(*J -E"')*( K(* $#%% =#%L$')*( %#33(

To convert from molarity to percent solution-E

% solution = molarity * FW xxxxxxxxxx10 !I.7%%%2* -E#IGI

K%%%2*

$=#IGI $L$#%"%%GJquotesdbs_dbs14.pdfusesText_20
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