[PDF] Antitumor Effects of Ricin AChain Immunotoxins Prepared from Intact





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La Ricine une arme biologique : « Info ou In…tox ?! »

Etes-vous de la génération « huile de ricin » ? En réalité ce n'est pas loin de la vérité



CONDUITE A TENIR LORS DUN ÉVÉNEMENT BIOLOGIQUE OU

07?/05?/2019 La fabrication et la volonté des terroristes à utiliser des armes ... tutoriels de fabrication d'explosifs et de ricine (toxine) lors d'une.



FICHE DE RENSEIGNEMENTS SUR LE RICIN

Il faudrait délibérément fabriquer du ricin et l'utiliser sciemment pour empoisonner quelqu'un. De même l'exposition involontaire au ricin est très peu probable 



Comment faire des polyamides à partir de lhuile de ricin ? - Du ricin

ne doit pas faire oublier que l'huile de ricin est à la base aujourd'hui



Antitumor Effects of Ricin AChain Immunotoxins Prepared from Intact

and Fab' Fragments on Solid Human Hodgkin's Disease Tumors in Mice1 to deglycosylated ricin A chain (dg A) to evaluate their potential as.



Fabrication of a SERS based aptasensor for detection of ricin B toxin

02?/11?/2014 Fabrication of a SERS based aptasensor for ... Ag nanoparticles labeled with Bpy and ricin B aptamer which act as SERS probes.





Conduite à tenir lors dun événement biologique ou chimique

19?/04?/2019 La fabrication et la volonté des terroristes à utiliser des armes ... tutoriels de fabrication d'explosifs et de ricine (toxine) lors d'une.



Attentat au moyen de toxines

ricin 84 mg de ricine et des ustensiles nécessaires à la fabrication d'un explosif sont saisis. Il est reproché à l'islamiste et sa femme d'avoir planifié 



CONDUITE A TENIR LORS DUN ÉVÉNEMENT BIOLOGIQUE OU

Les criminels et les terroristes ont démontré leur capacité à fabriquer des mai 2018 :découverte de tutoriels de fabrication d'explosifs et de ricine.



Ricine et risque biologique - ResearchGate

La ricine est une protéine hétérodimérique constituée de deux chaînes A et B de 31 et 34-37 kDa respectivement liées par un pont disulfure [23] (Figure 1) Elle est synthétisée sous la

How to prepare ricin?

Wear a gas mask and gloves. Try to keep the ricin wet at all times to avoid generating any dust (DEADLY!). And always shower and change clothes after handling. 5.Preparation Ricin is a protoplasmic poison prepared from castor beans after the extraction of castor oil therefrom.

How is ricin extracted from Iranian Ricinus communis?

This study undertook extraction and identification of ricin, a toxalbumine, from Iranian Ricinus communis L. Ricin, was extracted from the seeds using dilute acid solution, salted out with ammonium sulfate, and purified by Sephadex G - 75 and DEAE - cellulose column chromatography. Disc electrophoresis showed the degree of the purification.

What is ricin protein?

Introduction Ricin is a highly toxic protein which can be extracted from the castor bean seeds (Ricinus communisL.). This plant is present in all Brazilian regions and explored commercially for its oil, which is mainly used for the production of lubricants, fuel and drugs.

How is ricin precipitated from the water extraction step?

The filtrate from the water extraction step, which contains the ricin, was treated with a 16.7% solution of Na SO to precipitate the protein. This solution is com- "ice posed of 20 pounds of salt in 100 pounds of water and the amount used was such that the salt content equalled 20% of the filtrate weight.

Antitumor Effects of Ricin AChain Immunotoxins Prepared from Intact [CANCERRESEARCH50,2929-2935.May15.1990]

ABSTRACT

diseaseinhumans.

INTRODUCTION

Received11/13/89;revised2/9/9Ãoe.

Hodgkin'sdiseaseinhumans.

MATERIALSANDMETHODS

antibody. usingthePharmaciaPharmphastsystem. niquesasdescribedelsewhere(17).

2929Downloaded from http://aacrjournals.org/cancerres/article-pdf/50/10/2929/2439636/cr0500102929.pdf by guest on 01 October 2023

%blocking= cpminthepresenceofblockingantibody. fortheexperiments. fragments. wascalculatedasfollows

Volume=d2-D--

6 insignificant. analyseshasbeendescribedpreviously(7).

RESULTS

Cross-BlockingofHRS-3,Ber-H2,andIRac

100
tions.

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vivotomaximizetumorcellkill. beenpublishedpreviously(7,12,13).

CytotoxicitytoL540CellsinVitro

Immunotoxin1C»(M)

TissueHRS-3Ber-H2IRac

Adrenal

Brain(cortex)

Brainstem

Breast

Cerebellum

Cervix

Colon

Esophagus

Gallbladder

Heart

Kidney

Liver Lung

Lymphnode

Mucosa(nasal)

Ovary

ParathyroidPros'-'te

Spleen

Stomach(antrum)

Stomach(body)

Testis

ThyroidThyroid(AI)*Thyroid(Hashimoto's)

Tonsils

Uterus

Vagina

Hodgkin'sdisease0

ftAI,autoimmunethyroiditis. cPrimarymaterialandcelllines.Ber-H2dgA

HRS-3-dgAHRS-3Fab'dgA

9.0±0.8x10'"

7.0±1.5X10-'"

1.0±0.2x10""

6.0±1.2x10-"'

immunotoxin.

AntitumorEffectsonSolidL540TumorsinVivo

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Treatmcn!PBSPBSIRac-dgAIRac

dgAIRac dgAIRacIRac

Fab'dgAIRac

Fab'HRS-3

dgAHRS-3HRS-3

Fab'dgAHRS-3

Fab'Ber-H2

dgABer-H2OX?. (Day30/Day1 ratio)9.7

±1.6*5.8

±0.70.0

±0.00.8

±0.20.9

±0.47.5

±1.58.0

±2.910.6

±1.91.4

±0.57.5

±1.82.7

±0.77.9±1.54.6

±1.88.8

±1.78.7

±1.1No.of

72
57684
51
63
38578
*Mean±SE. cAverageof3separateexperiments.

Averageof2separateexperiments.

0.8I mE o>E _3 O h.OEa)IgGimmunotoxins o 0.81

0.4•

0.01020

b)IgGantibodies

1O2030

Daysaftertreatment

0.4- CO 0.4b)Fab'fragments

0.0O102030

Daysaftertreatment

tumoreffect(Fig.3A).

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ofTreatment

3;Fig.4).

102030

Daysaftertreatment

group:bars,SE.

ImmunotoxinsensitivitvSubline1234L

xIO'"4 x10-'°6 xIO"102 x10-'1 xIO'"M)HRS-3dgA1 x10-6x 10-1 x10-4x

10-0t009x

IO'' tumorcellescape. anttotheimmunotoxins.

DISCUSSION

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cocktails.

ACKNOWLEDGMENTS

REFERENCES

Semin.Oncol..13(Suppl.1):23-26.1986.

disease.J.Clin.Oncol..5:556-561,1987. ease.Br.J.Cancer,59:631-635,1989. plantation.Blood,73:340-344.1989.

Int.J.Cancer.26:723-731,1980.

1986.
celllines.J.Immunol.,139:2081-2087.1987.

20,1980.

1987.

Biochem..160:440-443,1987.

2934Downloaded from http://aacrjournals.org/cancerres/article-pdf/50/10/2929/2439636/cr0500102929.pdf by guest on 01 October 2023

CancerRes..47:5042-5046.1987.

4428.1983.

1984.
model.CancerRes..48:4862-4867.1988.

Acta.96Â":172-178.1988.

antibody.

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quotesdbs_dbs31.pdfusesText_37
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