[PDF] 10x to 2x dilution

You need to make a 1:5 dilution of a solution. You need 10 ml of the diluted solution How do you make a 500 ml solution of 2x TBE buffer from the 10x buffer?Autres questions
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  • How to do a 2X dilution?

    Calculate the volumes of stock solution and water needed to dilute the stock solution. Each diluted solution should have a total volume of 1 mL or 1000 ? L. In the left column in the table below, the example solution has a dilution factor of 2X meaning you will add 500 ? L of stock solution to 500 ? L of water.

  • How do you dilute 10x to 1X?

    To make 1 liter of 1x buffer, measure 100 mL of 10x stock and add 900 mL water to make the total volume equal to 1000 mL (1 liter).

  • What is 2X dilution factor?

    A two-fold dilution reduces the concentration of a solution by a factor of two that is reduces the original concentration by one half.
  • Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. The final volume of the diluted sample is 1000 µL (1 mL), and the concentration is 1/10 that of the original solution. A 1:10 dilution is also called a 10x dilution.
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