Seeing the Trees through the Forest Analytical Challenges and
Effect of dilution is not always linear a 10x dilution can result in greater 2 – 2x dilution pre-cleanup : 10x dilution post cleanup.
Lab Calculations Units
Some stock solutions are concentrated and need to be diluted before using. • Form example a 10X stock solution is one that contains ten times the concentration
IFU Template
23 juil. 2009 Ventana Medical Systems' (Ventana®) 10X SSC Sodium Chloride Sodium Citrate ... Once diluted
Buffer TTGB 10X - Reference
The Buffer TTGB 10X is a binding buffer that enhances the. Apolipoprotein H (ApoH) protein Medium size sample (0.2-5.0 mL): dilute Buffer TTGB 10X to 2X.
Sample Preparation Kit Pro MANUAL
Reduction Stock Solution. 2x 0.5 ml tube orange cap. LC Solution. 4x 10 ml tube
Trypsin/EDTA (10x solution)
Once thawed aseptically dilute 100ml of the 10x concentrated solution with 850ml of a sterile. Ca2+- and Mg2+-free salt solution (e.g. Dulbecco´s PBS) and mix
Thermo Fisher Scientific
Dilute the 10X TNE ten (10) fold to obtain a 1X TNE work- ing buffer solution by transferring Prepare dsDNA standard dilutions and unknown samples at 2X.
M3013 The Dilution Solution - Students Guide Introduction: Have
This could also be called a ten-fold dilution or a “10X” dilution because the table below
SolisFAST™ SolisGreen® qPCR Mix (no ROX) 5X FIREPol® DNA
2x or 3x solution). 10x Solution S is NOT a reaction buffer and should be used ONLY IF non- specific amplification occurs. Additional reagents required:.
PBS Plus 10X
Mix 1 part concentrated buffer to 9 parts deionized water. (1:10 dilution). -OR-. Dilute contents of the 10X PBS Plus bottle (500 ml) to 5
Rick Jordan
Lilly Asanuma
Pacific Agricultural Lab
Sherwood, OR
Challenge of Residue Analysis in Difficult
Matrices
Agilent User Meeting NACRW
Naples,FL,July27,2017
Co-extracted materials
Matrix effects (suppression / enhancement) will complicate accurate quantitation of target compoundsCan cause issues with sample introduction systems
MS/MS source contamination
Potential Solutions
Reduction in initial sample amount
Will have a negative impact on LOQ if calibration range is not adjustedDilution of sample extract
Effect of dilution is not always linear, a 10x dilution can result in greater than 10x reduction in background matrix levelsWorks well for large multi-residue methods
Use of d-SPE cleanups
Can target specific matrix interferences
Agilent User Meeting NACRW
Naples,FL,July27,2017
Difficult Matrices
Agilent User Meeting NACRW
Naples,FL,July27,2017
BoswelliaPowder (anti-inflammatory)
Olive Powder
Spirulina (blue green algae)
Experimental
Agilent User Meeting NACRW
Naples,FL,July27,2017
Evaluate cleanup and dilution schemes for matrix removal3 matrices
4 different cleanup / dilution schemes
140 compounds
Pesticide recovery trends for each technique
Experimental
Agilent User Meeting NACRW
Naples,FL,July27,2017
QuEChERSExtraction (AOAC 2007.01)
5g sample hydrated with 17mL water for 30 minutes
15mL extraction solvent
d-SPE cleanup Fatty Samples d-SPE (5982-5122)50mg PSA
50mg C18EC
150mg MgSO4
Experimental
Agilent User Meeting NACRW
Naples,FL,July27,2017
Cleanup and dilution schemes
1 no dilution, 20x dilution post cleanup
2 2x dilution pre-cleanup : 10x dilution post cleanup
3 4x dilution pre-cleanup : 5x dilution post cleanup
4 10x dilution pre-cleanup : 2x post cleanup
Total dilution factor of 20x for all injections
Agilent User Meeting NACRW
Naples,FL,July27,2017
Olive Powder
no dilution2x dilution
4x dilution
10x dilution
Agilent User Meeting NACRW
Naples,FL,July27,2017
Spirulina
no dilution2x dilution
4x dilution
10x dilution
Agilent User Meeting NACRW
Naples,FL,July27,2017
Boswellia
Black-no cleanup 20x dilution
Red-STR pre-cleanup 20x post cleanup
Green-10x pre-cleanup 2x post cleanup
Agilent User Meeting NACRW
Naples,FL,July27,2017
Black-no cleanup 20x dilution
Red-STR pre-cleanup 20x post cleanup
Green-10x pre-cleanup 2x post cleanup
Olive Powder
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 2 4 6 8 10 12Algae Powder
20X2X + 10X4X + 5X10X +2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 2 4 6 8 10 12Boswellia
20X2X+10X4X+5X10X+2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 2 4 6 8 10 12 14Olive Powder
20X2X+10X4X+5X10X+2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 2 4 6 8 10 12Algae Powder
20X2X+10X4X+5X10X+2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 2 4 6 8 10 12 AbamectinAcequinocyl-HydroxyClethodimPropamocarbSaflufenacilSethoxydimSpinetoram JSpinosyn DBoswellia
20X2X+10X4X+5X10X+2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 2 4 6 8 10 12 14 16 18Boswellia
20X2X+10X4X+5X10X+2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 10 20 3040
50
60
Boswellia-Fenpropanthrin
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 2 4 6 8 10 12Olive Powder
20X2X+10X4X+5X10X+2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 5 10 15 20 25Olive Powder
20X2X+10X4X+5X10X+2X
Agilent User Meeting NACRW
Naples,FL,July27,2017
0 10 20 3040
50
60Olive Powder Formentanate-HCl
Agilent User Meeting NACRW
Naples,FL,July27,2017
Takeaways
Use fullscan
techniquesAll dilutions are not created equal
Too muchmatrix on sorbents can negatively impact compound recovery Too littlematrix on sorbents can negatively impact compound recovery Evaluate dilution schemes to measure amount of matrix removed and compound recovery Difficult or challenging matrices may have different optimal schemesAgilent User Meeting NACRW
Naples,FL,July27,2017
Acknowledgements
quotesdbs_dbs31.pdfusesText_37[PDF] 11 111 meaning angels
[PDF] 11 111 meaning twin flame
[PDF] 11 2 study guide and intervention rational functions
[PDF] 11 3 skills practice probability distributions answers
[PDF] 11 4 skills practice multiplying and dividing rational expressions answers
[PDF] 11 4 study guide and intervention multiplying and dividing rational expressions answers
[PDF] 11 5 practice solving rational equations
[PDF] 11 5 study guide and intervention dividing polynomials
[PDF] 11 7 study guide and intervention mixed expressions and complex fractions
[PDF] 11 7 study guide and intervention trigonometric ratios
[PDF] 11 8 skills practice rational equations answers
[PDF] 11 alive news anchor faith
[PDF] 11 alive news anchor fired
[PDF] 11 alive news anchor vinny
