RÉSUMÉ
Dilution 1 : diluer les échantillons de plasma ou de sérum de 1:100 avec le diluant pour échantillon de complément (échantillon de 10 µl + 990 µl de.
Protocole de dilution
Vous devez effectuer des dilutions uniquement lorsqu'une valeur de test se situe hors Préparation d'une dilution manuelle ou automatisée ... 1 (100 µL).
1. dilutions and concentrations. liquid and solid samples
This situation requires the dilution or concentration of the sample prior to carry 1:100 dilution is needed (or the 10-2 dilution) it can be prepared ...
DILUTION CHART.pdf
MIXTURE. Dilution Chart. 1:1. 1:5 1:10 1:20 1:25 1:50 1:100 1:150 1:200 1:250 1:500 1:1000. 500ml 200ml 100ml 50ml.
dilution.pdf
Dilutions et dilutions infinitésimales dynamisées ! ... dilution au 1/2 1/5
Réalisation de dilutions sur lanalyseur de biochimie IDEXX Catalyst
Réalisation de dilutions sur l'analyseur de biochimie de biochimie Catalyst One* permet de réaliser des dilutions automatisées (il ... 1 (100 µl).
CALCULS CONVERSION DUNITES
10?/11?/2005 CONVERSIONS D'UNITES. • TABLEAU DE CONVERSIONS D'UNITES. • CALCULS DES DILUTIONS. • UNITES DE MASSE. • UNITES DE VOLUME. • LA REGLE DE TROIS.
Solvants solutés et règles de dilution
eau PPI dilution possible ensuite dans G5% ou NaCl 0
SALMONELLA SEROLOGY
agglutinines anti-O et anti-H spécifiques par dilution des sérums de patients. La dilution finale des échantillons est alors de 1/100 à 1/1600 pour ...
Tableau de dilution
Lors de la dilution de vos produits chimiques vous trouverez qu'il est utile de remplir votre contenant avec la quantité TAUX DE DILUTION ... 1:100.
Microbiology BIOL 275 DILUTIONS - University of Pennsylvania
To make the 1/100 serum in buffer dilution use the first dilution as a starting point and repeat the procedure in step 1 above; i e place 1 part of the 1/10 diluted serum in a vessel and bring the volume up to 10 parts total
HOW TO SOLVE PRACTICAL ASPECTS OF MICROBIOLOGY - OCW
1:10 dilution = 10 ml of 1:10 dilution (or 10-1 dilution) 1 ml of sample + 9 ml of diluent If a higher dilution of the sample is required successive dilutions can be prepared For example if the 1:100 dilution is needed (or the 10-2 dilution) it can be prepared adding 1 ml of 1:10 dilution to 9 ml of diluent according to the following
Dilution factor: 10X
Final volume = 1000 mL (1 L) Dilution factor: 10X Stock volume: 1000 mL/10 = 100 mL Now you need to calculate the amount of water you will add the 100 mL stock solution to Do this by subtracting 100 mL from the final volume: Final volume: 1000 mL Volume of water to add: 1000 mL – 100 mL = 900 mL
What is a 1 1000 dilution?
What is the dilution factor of 1 1000? You could make 1/1,000 by adding 1 microliter of sample to 0.999 ml diluent. Why is that a poor choice? Because you can’t measure 1 microliter (or even 10 microliters) accurately with ordinary pipeters. So, make three serial 1/10 dilutions (0.1 ml [100 microliters] into 0.9 ml): 1/10 x 1/10 x 1/10 = 1/1,000.
How do you do a 100 fold dilution?
How do you do a 100 fold dilution? For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent. Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. The final volume of the diluted sample is 1000 µL (1 mL), and the concentration is 1/10 that of the original solution. How do you fold a dilution?
What are examples of dilution in chemistry?
What are examples of dilution in chemistry? A dilution is a solution made by adding more solvent to a more concentrated solution (stock solution), which reduces the concentration of the solute. An example of a dilute solution is tap water , which is mostly water (solvent), with a small amount of dissolved minerals and gasses (solutes) .
HOW TO SOLVE PRACTICAL
ASPECTS OF MICROBIOLOGY
1. DILUTIONS AND CONCENTRATIONS. LIQUID AND SOLID SAMPLES
Inés Arana, Maite Orruño & Isabel Barcina
Department of Immunology, Microbiology and ParasitologyUniversity of the Basque Country
Universidad del País Vasco (UPV/EHU)
OCW 2013
1. DILUTIONS AND CONCENTRATIONS. LIQUID AND SOLID
SAMPLES
In most environments, the microbial density is usually too high or too low to obtain good results in the enumeration of microorganisms by a direct culture of the sample. This situation requires the dilution or concentration of the sample prior to carry out any study. Additionally, solid samples must be diluted for an easier handling, in this way; they can be treated as liquid samples. In most cases, we work with decimal dilutions. The simplest case is the preparation of 10 ml of the1:10 dilution of the sample. For this, 1 ml of sample is added to 9 ml of diluent; consequently, in 10 ml
of this 1:10 dilution, 1 ml corresponds to the sample. Expressing by an equation:1 ml of sample
1:10 dilution = 10 ml of 1:10 dilution (or 10
-1 dilution)1 ml of sample + 9 ml of diluent
If a higher dilution of the sample is required, successive dilutions can be prepared. For example, if the
1:100 dilution is needed (or the 10
-2 dilution), it can be prepared adding 1 ml of 1:10 dilution to 9 ml of diluent, according to the following equation:1 ml of 10
-1 dilution1:100 dilution = 10 ml of 1:100 dilution (10
-2)1 ml of 10
-1 dilution + 9 ml of diluent Or directly, adding 1 ml of sample to 99 ml of diluent:1 ml of sample
1:100 dilution = 100 ml of 1:100 dilution (10
-2)1 ml of sample + 99 ml of diluent
Or adding 0.1 ml of sample to 9.9 ml of diluent:
0.1 ml of sample
1:100 dilution = 10 ml of 1:100 dilution (10
-2)0.1 ml of sample + 9.9 ml of diluent
Note: The final volume obtained is given by the denominator of the equation. With these notions, you must propose solutions to the following problems:1.1. How would you prepare 250 ml of a 10
-1 dilution from a water sample?1.2. How would you prepare 10 ml of a 10
-5 dilution in 3 steps?What if the sample is not liquid, for example, a food sample? In this case, we can consider that 1 gram
of sample is equal to 1 ml:1 g of sample
1:10 dilution = 10 ml of 1:10 dilution (10
-1)1 g of sample + 9 ml of diluent
1.3. 1.5 grams of food were added to 13.5 ml of Ringer Solution (diluent) and homogenized. What is
the resultant dilution? As previously indicated, sometimes, due to the low density of microorganisms, it is necessary toconcentrate the sample by filtration or centrifugation. This fact requires for subsequent handling, the
resuspension of the microbes retained in the filters or accumulated in the pellets in a given volume of
diluent. In this case, it is necessary to determine the concentration factor. For example:10 ml of filtered sample
Concentration factor = 1 ml 10X concentrate
1 ml of diluent
1.4. 100 ml of a water sample were filtered, and then the filter was suspended in 10 ml of saline
solution and shaked vigorously. What is the concentration factor?1.5. 100 ml of a dense microbial suspension were centrifuged at 5,000 rpm. The supernatant was
removed and the pellet was resuspended after adding 2.5 ml of diluent. What is the concentration factor? We must know not only how to dilute or concentrate a sample; we must also understand how to properly apply the dilution or concentration factors to determine the microbial density of a sample. Here are some problems based on the enumeration of microorganisms by the method of counting colony forming units (CFU).A colonies
CFU/ml = X Dilution FactorB volume plated (ml)
A colonies 1 CFU/ml = X B volume plated (ml) Concentration Factor1.6. We have prepared the followings dilutions from different sample: 1:10, 1:5, 1:4 and 1:2. Then,
0.1 ml of each dilution is plated. After incubation, in all cases 27 colonies grew in the plates,
which are the microbial densities of the samples?1.7. A food sample was processed following this protocol:
· 15 g of food were added to 135 ml of Ringer solution and homogenized. · 2 ml of the suspension were mixed with 18 ml of diluent and homogenized. · 0.2 ml were plated on nutrient agar and incubated.· 100 colonies were counted in the plate.
From this information, how many microorganisms were present per gram of food?SOLUTIONS
225 ml
SAMPLE
1.1. 250 ml of a 10-1 dilution
25 ml sample/(25 ml sample + 225 ml diluent)
1.2. 10 ml of a 10-5 dilution in 3 steps
There are other options, for example:
25 ml1 ml 0,1 ml 0,1 ml
SAMPLE
9 ml 9,9 ml 9,9 ml
10-1 10-3 10-5
1:10 1:10 x 1:100 1:1,000 x 1:100
1:1,000 1:100,000
0,1 ml 0,1 ml 1 ml
SAMPLE
9.9 ml 9.9 ml 9 ml
10-2 10-4 10-5
1:100 1:100 x 1:100 1:10,000 x 1:10
1:10,000 1:100,000
27 colonies x 101
1:10 dilution: = 2.7 10
3 CFU/ml
0.1 ml plated
27 colonies x 5
1:5 dilution: = 1.35 10
3 CFU/ml
0.1 ml plated
27 colonies x 4
1:4 dilution: = 1.08 10
3 CFU/ml
0.1 ml plated
27 colonies x 2
1:2 dilution: = 5.4 10
2 CFU/ml
0.1 ml plated
100 colonies x 10 x 10
= 5 104 CFU/g
0.2 ml plated
1.3. 1.5 g food + 13.5 ml diluent. Dilution?
1.5 g sample/(1.5 g sample + 13.5 ml diluent) = 1,5/15 = 1/10
1.4. 100 ml filtered and resuspended in 10 ml of diluent. What is the concentration factor?
100 ml sample/10 ml diluent = 10101010
1.5. 100 ml centrifugated and resuspended in 2.5 ml of diluent. Concentration factor?
100 ml sample/2.5 ml diluent = 44440000
1.6..1:10, 1:5, 1:4 and 1:2 dilutions. 1 plate/dilution. 0.1 ml/plate. 27 colonies/plate. CFU/ml?
1.7. 15 g sample + 135 ml diluent = 15:150 dilution = 1:10
2 ml 1:10 dilution + 18 ml diluent = 2 :20 dilution = 1 :10
Volume spread = 0.2 ml. Colonies/plate = 100 colonies. Microorganisms/g food?quotesdbs_dbs35.pdfusesText_40[PDF] medicaments urgence reanimation
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