[PDF] Centrifugation and addition of glycerol at 22 °C instead of 4 °C





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Centrifugation and addition of glycerol at 22 °C instead of 4 °C

Jun 1 2020 Only ejaculates with more than 60.106 spermatozoalmL and 50% motile sperm after dilution were used. Extenders. The basic extender used for these ...



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Dec 3 2019 teaching and research institutions in France or ... Laboratory centrifuge for 50-ml tubes: Sigma 4-16 K (or equivalent). • Cell culture hood.



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CENTRIFUGATION

M. lNational

Received

ABSTRACT

The +2%+2.5% (0

Keywords:

INTRODUCTION

Sperm(1,20,

Elimination

Acknowledgments.

1.Couty

A.Mouret

aliparticipating adjustment (7, 1

During

-0C/min The 1h, 1h

MTERIALSME1HODS

Extenders.

Freezing

Temperature

centrifugation/glycerol Ali

Freezing

Unique

Addition

Motility

Table

Modified

Skim

Glucose,

25
L 0.5L 505o
6.8 t a

Temperature 0C

0+Time

045
b

Temperature 0C

40 .
35
Long 30
25.
20

Control

Slow -----Long 1 1à CGFC 5. 0+

0456Time

Figure

Experiment

The +2

Theoin

The The The For

Experiment

T in

Experiment

The This The

Statistical

For For

RESULTS

Table1.First,

V a 4.5 5 by Table

Motility

1 centrifugation b4 Table

Motility

significantly

Experiment

Mean

11mls±

Experiment

The

Thirteen

Table

10minutes

B56c63

E67 G58

H867119

451163

K50633

M176506

TOTAL243

1(pregnant

DISCUSSION

In

Differences

In (7,1

Changing

It extender&Nguekam Ithas

Iaddition,

Ifincreased

Glycerol

(7)compared When In reported At The

REFERENCES

1. 0F

JI,Fahim

O. Fer

C.Factors

14. 34.
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