[PDF] IHC-Amplified Fluorescent Frozen Sections

What is the protocol for frozen immunohistochemistry (IHC-Fr) sections?

Discover moreat abcam.com/technical Immunohistochemistry (IHC-FR)-Frozen sections protocol Frozen sections: Once mounted on APES coated slides, frozen sections are best kept at -80°C until needed. 1. When required, leave to warm at room temperature for 5 mins. 2. Pre cool the fixative (acetone, methanol or ethanol) at -20°C for 30 mins.

What is the IHC protocol?

The following immunohistochemistry (IHC) protocol has been developed and optimized by R&D Systems’ IHC/ICC laboratory for chromogenic IHC experiments using frozen tissue samples. This IHC protocol provides a basic guide for the fixation, cryostat sectioning, and staining of frozen tissue samples.

How to incubate frozen tissue sections for chromogenic IHC staining?

For chromogenic IHC staining of frozen tissue sections using R&D Systems antibodies, it is recommended to incubate overnight at 2-8 °C. This incubation regime allows for optimal specific binding of antibodies to tissue targets and reduces non-specific background staining.

What is the ICE protocol?

The ice protocol consisted of a 10 minute water immersion, superior to the knee joint, at 10°C. Range of movement (ROM), using a standard plastic goniometer, and soreness, using a Visual Analogue Scale (VAS), were assessed. Measurements were taken prior to, and 48 hours post exercise. Results were analyzed using a related t-test.