(MFI of the 0-ng/mL standard point) might increase and the overall assay Pipette 500 µL of Assay Diluent into each of the Continue making serial dilutions by transferring 500 µL Note: You can use the same calculations for the number
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[PDF] Serial Dilutions
Also called a “dilution series”, serial dilutions are, essentially Problem: Make 10 mL of a 1 ng/mL To make this solution, you would need to add 0 001 μL of GFP stock to enough water do is calculate how to make the individual dilutions
[PDF] Laboratory Math II: Solutions and Dilutions - NIH Office of Intramural
If molarity or normality, the molecular or formula weight of the Slide 24 Serial Dilutions: How It Looks 24 5μl 2μl Stock [3 45mM] 10μl [1mM] [10μM] [100nM ]
[PDF] Download - PREXEL-Q_entire_procedurepdf - Gene Quantification
15 déc 2005 · File for tabulating exact primer and probe sequence data, calculating This Test Plate dilution series thus represents: 20 85351 ng/uL in well
[PDF] Download Manual - BD Biosciences
(MFI of the 0-ng/mL standard point) might increase and the overall assay Pipette 500 µL of Assay Diluent into each of the Continue making serial dilutions by transferring 500 µL Note: You can use the same calculations for the number
[PDF] PicoGreen Assay
Step 1: Calculate # of samples and prepare 1X TE: Use 2μL of stock λ-DNA of 100 ng/μL (provided) + 18μL of 1X TE = 20μL of 10 ng/μL working solution, Make five serial dilutions using 2 ul working solution into 18 ul TE to make the
[PDF] Lab 3 - B75 Lab manual_ss_pt1
µg/µL micrograms per microliter ng/mL nanograms per milliliter ng/μL concentration, use the equation that follows Mass/Volume Concentration Equation: Prepare a 1:10 serial dilution of the concentrated (300 mg/mL) stock according to
[PDF] Dilutions Occasionally a solution is too concentrated to be used as it
use in calculating the concentration of solutions in a series is to multiply the original used in reverse to produce a dilution series having prescribed concentrations at each 1 µg = 1000 ng 1 ng = 1000 pg 1 liter = 1000 ml 1 ml = 1000 µl 4
[PDF] Mathematics Exercises in Biotechnology
11 fév 2005 · 22 Test for Endotoxin: A Serial Dilution Problem 48 (b) The next step requires calculating the number of µL of enzyme required per digest (b) 5 × 103 colonies per µg = 5000 colonies per µg or 5 colonies per ng Since a
[PDF] Biotech 2 – Making Serial Dilutions
A serial dilution is a series of dilutions in which a concentrated stock solution is Calculate the volumes of solute (what you are diluting) and diluent (what you are 900 μl H 2 O 1 μg/μl stock 1 ng/μl 0 1 ng/μl 10 -2 ng/μl 10 -3 ng/μl 10 -4
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