[PDF] PREPARING SOLUTIONS AND MAKING DILUTIONS PDF solutions.pdf

For example, a 1:5 dilution (verbalize as "1 to 5" dilution) entails combining 1 unit volume of diluent (the material to be diluted) + 4 unit volumes of the solvent medium (hence, 1 + 4 = 5 = dilution factor)

5 Dilute the serum 10 times with saline 6 Make a dilution of 1 part serum use in calculating the concentration of solutions in a series is to multiply the original

[PDF] Laboratory Math II: Solutions and Dilutions - NIH Office of Intramural

Slide 1 Laboratory Math II: Solutions and Dilutions Philip Ryan, PhD Post- Doctoral A five molar solution means we have five moles per liter And we want a However, we can use a series of smaller dilutions into smaller volumes to get to

[PDF] 261_Labs_Making Solutions and Dilutions - Penguin Prof Pages

For example, a 1:5 dilution (verbalize as "1 to 5" dilution) entails combining 1 A serial dilution is simply a series of simple dilutions which amplifies the dilution

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precise measurements, like diluting a chemical stock to make a series of calibration Dilution factor = (800 mL + 200 mL)/200 mL = 5 -> Your lab partner made a 5X dilution A ten-fold dilution is sometimes also written as a 1:10 dilution

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of these calculations requires a series of steps Understanding the taining 10 g of active ingredient with a strength of 20 or 1 : 5 w/v is diluted to 100 mL,

[PDF] DILUTION CHART NEW - Caprichem

DILUTION CHART 1:1 1:5 1:10 1:20 1:25 1:50 1:100 1:150 1:200 1:250 1:500 1: 1000 CHEMICAL 500ml 200ml 100ml 50ml 40ml 20ml 10ml 7ml 5ml 4ml 2ml

[PDF] Microbiologics Dilutions Guide

1 0 0 = 100 350 0 = 3 5 x 102 3 5 0 = 350 Microbiologics Dilutions Guide This document outlines how to perform dilutions when using Microbiologics

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NOTE:..

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How to Make Simple Solutions and Dilutions

Unit Definitions

milligram " gram " kilogram " mole "(%)'!#% molarity " molar "" eg)* millimole "#$#%%% millimolar "" eg)% 1. Simple Dilution (Dilution Factor Method).. simple dilution unit volume + solvent + ,dilution factor 0#*0 12 #12"*" )33 4 050
667
)%5 8 050
!).#5

667)%5667

)%5667#3

2. Mixing parts or volumes..

0# 0! 9 7 !.4 8 7 :4 0000 #4

3. Serial Dilution..

serial dilution /etc , 4 total dilution factor product -;- ";-#=;-)=;-'etc !.4 >4??@9)%# three step #.#%% ##%A 33

33%A "#.#%%4!

of the 1:100 dilution 33
#.#%%=#%%"#.#%%%%B less

4. Making fixed volumes of specific concentrations from liquid reagents: V1C1=V2C2..

CD eg

V = volumeC = concentration;

V1C1=V2C2

!#.6 $"C1 )%%A"C2 $"V2 9 V1 )%%A V1 " D

This is your unknown.

C1 "#%%

V2 "")%%A"%)

C2 "")*

C #"C)!7) $7# C "%%*"*%A 6*%A #*%A A)*

5. Molar solutions (unit = M = moles/L)..

6 molarity +#formula weight -E molecular weight !#.To prepare a liter of a simple molar solution from a dry reagent: formula weight E

7-E"#32'

$F%#* #32' $")3#2* !).To prepare a specific volume of a specific molar solution from a dry reagent: -E#G% 8 H $ =-E H $ =-E H "%%)*=%#*$=#G% H "%(I*

6)%#

%(I*

6. Percent Solutions (= parts per hundred)..

percent concentrations. E dry mass (g) per volume H $#%%"#%J<7 <7 #%J$<7 !#.4'J$<7 <7#%% 8 #J$ !).,'*%#)J$<7 !'*"2) 62)
<7'*% !'.#*%%*J$ %%*=#*$#%% "%%%I* I*

6%%%I*

E liquid reagents volume per volumeieH !2.4I%J$!I%#%%J !*.,3#))#*J$,5&#%% )#*=3#)$#%% "#3(#

6!#3(#,5&#%%I#3)#J$

7. Conversions from % to molarity and from molarity to %..

To convert from % solution to molarity

Molarity = (% solution) * 10

Xxxxx FW

!(.,(*J -E"')*( K(* $#%% =#%L$')*( %#33(

To convert from molarity to percent solution-E

% solution = molarity * FW xxxxxxxxxx10 !I.7%%%2* -E#IGI

K%%%2*

$=#IGI $L$#%"%%GJquotesdbs_dbs14.pdfusesText_20

[PDF] [PDF] PREPARING SOLUTIONS AND MAKING DILUTIONS