SA assay uses the coupling of antigens and antibodies and chorionic gonadotropin in urine with horseradish peroxidase (an d) Adding Test Sample Primary Antibody - The test sample of serum
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Enzyme-Linked ImmunoSorbant Assay (ELISA) - OHSU
SA assay uses the coupling of antigens and antibodies and chorionic gonadotropin in urine with horseradish peroxidase (an d) Adding Test Sample Primary Antibody - The test sample of serum
Teacher Background Using ELISA to Measure - OHSU
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ELISA Immuno Explorer Kit - Bio-Rad
tands for enzyme-linked immunosorbent assay This powerful antibody- based allergens and molecular markers of pregnancy and drug use Protocol III: ELISA Antibody Test
AUTOIMMUNITY
yme-linked immunosorbent assay (ELISA) is used to detect the presence of an antibody or antigen
Colorimetric, Fluorescent, Luminescent Methods for - Corning
Cité 3 fois — assay and discuss three types of detection systems – colorimetric, The most common enzymes used as labels for ELISA are (i) horse- Sample components (hemoglobin, bilirubin, cellular debris, drugs)
10 Drug Testing 2014
sts either detect or measure the concentrations of drugs in blood, serum or urine Drugs complex 2 As with all immunoassays, specificity not assured so this type of assay may
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USING ELISA TO MEASURE CONCENTRATIONS
OF SEX HORMONES OVER MENSTRUAL CYCLE
IN FEMALES AND OVER LIFESPAN IN MALES
THIS WORK IS LICENSED UNDER A CREATIVE COMMONS
ATTRIBUTION-NONCOMMERCIAL-SHAREALIKE 4.0
INTERNATIONAL LICENSE.
Enzyme-Linked
ImmunoSorbant Assay (ELISA)
ELISA yELISA - an acronym for Enzyme-Linked ImmunoSorbent Assay. yThe ELISA assay is a widely used biochemical assay to detect in a sample the presence of and quantity of proteins, such as hormones and antibodies and bacteria or viruses. yThe ELISA assay uses the coupling of antigens and antibodies and relies on the specificity and affinity of antibodies for antigens. Specificity is the ability to discriminate among diverse proteins. Affinity is the ability to tightly bind to molecules. yOne can determine how much antibody is present by starting with an antigen, or one can determine how much antigen or hormone is present by starting with an antibody.What Are Antigens?
yAntigens are any foreign substance in the body. y$QPLJHQV LQŃOXGH ³QRP-VHOI´ PROHŃXOHV MQG ŃHOOV VXŃO MV a. foreign proteins b. viruses c. environmental pollutants and other foreign substances like asbestos, tattoo ink, and cigarette smoke d. bacteria and parasites (Protista, Fungi, Plantae, andAnimalia cells)
e. foreign transplanted tissue f. cancerous cellsWhat Are Antibodies and How Are They Produced?
yAntibodies are large glycoprotein molecules produced by B-lymphocytes during the humoral immune response to antigens introduced into the body. yLymphocytes include B-lymphocytes (B-cells) and T- lymphocytes (T-cells) which are white blood cells form from the hematopoietic (blood) stem cells in the bone marrow. yThe immune system is made of two parts ± humoral (antibody-mediated) and cellular (cell-mediated).Formation of B Lymphocytes and T Lymphocytes
http://creativecommons.org/licenses/by/4.0/ No changes were made.B Lymphocytes Mature in the Bone Marrow and T
Lymphocytes Mature in the Thymus Gland
http://creativecommons.org/licenses/by/4.0/ No changes made.Humoral (Antibody-Mediated) Immune System
yB-lymphocytes produce large glycoproteins called antibodies in response to antigens (any foreign substance) and then mark those antigens-antibody complex to be destroyed by the T-lymphocytes.
yEach B-cell makes its own distinct antibody in response to a specific antigen which comes in contact with it. Each antibody is designed to bind to a specific surface binding site or epitope on the antigen.
Structure of An Antibody
yOver 80% of human glycoprotein antibodies are in the immunoglobulin class IgG. They are shaped like a Y and are found in the blood, lymph, and intestine.
yIgG molecules have a molecular weight of 150,000 Daltons and are made of 2 long (heavy) chains coded from chromosome 14, and 2 short (light) chains coded from either chromosome 2 or 22, and then all connected by disulphide bonds.