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Cell-Surface Immunology - JSTOR

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We have studied the expression of five surface antigens in eight Burkitt's lymphoma cell lines during different phases of the cell cycle and in different growth 

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ICANCERRESEARCH46,1,March1986]

Washington,DC¡0.A.]

ABSTRACT

INTRODUCTION

suchasneuroblastoma(8,9). beenelucidated. 28).
conditions.

MATERIALSANDMETHODS

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Table1

Cell linesST 486
CA46 EW36 MC116

Namalva

Raji AK778

USâ€"+++++â€"

BL,SAâ€"++++â€"

UL,USâ€"â€"++â€"

BL,APR++++++

BL,APR++++++++++

BL,APR+++++++++

0K\/J2MHLA6no.++

â€"+++â€"++++++++45

â€"â€"+++++++++48

some(range)(46-50) (43-49) (46-47) (45-63)(45-47) (46-48)t(8q-,14q+): observedchangescouldbeexamined. oftheseexperiments. humansurfaceimmunoglobulins. flowcytometricanalysis.

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usingstandardizedgainandwindowsettings. artifacts.

RESULTS

days2and3,anddecreasedduringdays4and5. detected.

5relativetoday1isclearlydemonstrated.I

o200 8160
x 2100
1234S

DAYSOFCULTUREPERIOD163248

CHANNELNUMBER

tested. 1500-
1000-
500

A,AK778;A,Raji;C,Daudi.

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Â"1.5

46.2
30.8

15.4Day1

Day5

ControlDay1

ControlDay5

32

CHANNELNUMBER4864

cellsonday5.

FLUORESCENCEVSFLUORESCENCEVSCOUNTS

80r
8x

G,/GofractionFL1CH16:27•

SphasefractionFL1CH28:42•

Gj/MfractionFL1CH43:62-80

60

CHANNELNUMBER

Gz/Mcellshavingahighermeanfluorescence.

paredto^2MinG2/Mcells.

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Fig.6.Meanfluorescenceintensitiesof

SlgMandf>2Mfluorescencearecalculatedsep

aratelyfortheG,phaseandtheG2/Mphase duringacultureperiod.Bars,SE.Thelinesof values(SlgM:r=0.99;02M=0.85).Left,A representativeexperimentshowingSlgM expressionduringa5-daycultureperiod.Note tions.maintaininganapproximatelyconstant ratioofGz/M:Gifluorescence.Eachpointon sameexperiment.Thereisonlyaslightde linesofregression.Themeanfluorescenceval 30
m DZ 20 io 1040r
30
Gj/M

012;4'»

DAYSAFTERLASTREPLACEMENTOFMEDIUM012345

DAYSAFTERLASTREPLACEMENTOFMEDIUM

•50V.-

tov.- S 0 -ioov.J Bl increaseofimmunofluorescenceonday5. finalvalueof3.5onday5. achieved.

DISCUSSION

cellcycleandthegrowthphase.

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ofthecellsinallgrowthphases. expressionwithdeclininggrowthrate. surfaceantigen.

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REFERENCES

SIS.1982.

711,1984.

Press,1980.

Research.NewYork:RavenPress,1980.

Nature(Lond.),225:645-647,1970.

303-311,1981.

Inst.,53:913-920.1974.

Immunol.,7:786-791,1977.

J.Immunol.,4:327-334,1974.

Cancer(Phila.),23:64-79,1969.

culture.J.Clin.Pathol.,78:261-273,1965.

514,1981.

64:477-483,1980.

Sect.APathol.(Suppl.274),215-221,1981.

899-907,1977.

J.Nati.CancerInst.,64:465-476,1980.

Cytometry,2.337-343,1982.

212,1981.

Immunol.,726:626-631,1981.

J.Nati.CancerInst.,49:1599-1606,1972.

Proc.Nati.Acad.Sci.USA,68:566-569,1971.

Sci.USA,68.2584-2588,1971.

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