Biological Wastewater Treatment 2-1 Aerobic -Organisms related to waste water treatment- eukaryotic ① Advantages and Disadvantages of CASP
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Principles and recent trends in biological
wastewater treatment technologyTeam E-Kansai
GECTechnical Coordinator
ChuzoNishizaki
Outline of This Presentation
1.Basics of Microbiology
2.Biological Wastewater Treatment
2-1. Aerobic Treatment
2-2. Anaerobic Treatment
2-3. Facultative Treatment
2-4. Nitrogen and Phosphorus Removal
3.Recent Trends
4.Conclusion
1. Basics of Microbiology
-Organisms related to waste water treatment- eukaryotic organismBacteria
(Major player) actinomycete blue-green algaeArchaea
(Major player) plant protozoa algae mushroom yeast fungi animal is very related to the waste water treatment is not much related to the waste water treatment prokaryotic organism1. Basics of Microbiology
-Structure of Bacterial Cells- Resource : Murray PR, et al. "Medical Microbiology" (Artifact)1. Basics of Microbiology
-Bacterial Growth Conditions-Environmental
FactorsOutline
Water䞉The growth of bacteria is dependent on the existence of water. pH 䞉Most microorganisms grow best when the pH is around 7.ThiobacillusandFungi : 5 (best growing)
Temperature
(maximum growth)Mesophilic bacteria : 20oC -42oC
Thermophilic bacteria : 40oC -70oC
Extreme thermophilic bacteria : 70oC-100oC
NutrientsMacronutrients : C, O, H, N, S, P, K, Ca, Mg and Fe Micronutrients : Mn, Mo, Zn, Co, Cu, Ni, V, B, Cl, Na, Se, Si and WDissolved Oxygen
Obligate aerobic bacteria : Pseudomonas sp. etc. Obligate anaerobic bacteria : Clostridium perfringens etc. Facultative anaerobic bacteria : E.coli, Citrobacter, etc. Light䞉Thegrowth of photoautotrophic bacteria is dependent of light. qT=q20(1.07)T-20 T : Temperature(Υ) qT: Rate of substrate utilizationFlagella rotation
Active transport etc.
Source : Strathkelvin Instruments Ltd.
1. Basics of Microbiology
-Metabolism of Heterotrophic Bacteria-Respiration
Growth
O2 CO2 HeatEnergyMaintenance
New biomass
Catabolism
Anabolism
Carbon ingestion
fs0 fe0Note : fe0+fs0=1
1. Basics of Microbiology
-Glycolytic Pathway and Cell Respiration (Catabolism)-Aerobic Respiration
Fermentation
Source : David Sadava, H. Craig Heller et al. : Life :T he Science of Biology 8th Edition1. Basics of Microbiology
-Fixation of Carbon Dioxide (Anabolism)-Plants and blue-green algae,
photosynthetic bacteria is a photoautotrophic organism.Chemoautotrophic bacteria
use CO2as a carbon source like a photosynthetic organism and grow.CalvinBenson cycle is what is
known as a major cycle to assimilate the CO2as a carbon source in these autotrophic organisms.Resource : Candela Open Courses
Note : RuBisCOїRibulose 1,5-bisphosphate carboxylase/oxygenase1. Basics of Microbiology
-Typical fs0ȝ*)Values for Key Bacterial Types-Organism TypeElectron
DonorElectron
Acceptors
Carbon
Source
fs0 (d-1)Aerobic, HeterotrophsBODO2BOD0.68.4
DenitrifiersBODNO3-BOD0.54
Nitrifying AutotrophsNH4+
NO2-O2O2
CO2CO2
0.14 0.10 0.92 0.62Methanogensacetate
H2 acetate CO2 acetate CO2 0.05 0.08 0.3 0.5Sulfide Oxidizing AutotrophsH2SO2CO20.21.4
Sulfate ReducersH2acetate
SO42-SO42-CO2acetate
0.05 0.08 0.29 0.5Fermenterssugarssugarssugars0.181.2
ʅ*)䠖maximum specific growth rate
Source : Bruce E. Rittmann, Perry L. McCarty : Environmental Biotechnology: Principles and Applications
2. Biological Wastewater Treatment
-Aerobic Treatment-Oxygen
DemandReactor TypesPopular NamesEffective MicroorganismsAerobic
Suspended
growth 䐟Conventional activated sludge process (CAS)Activated sludge-related
bacteriaPseudomonas
Flavobacterium
Corynebacterium
Aeromonas
Zoogloea
Acinetobacter, ...
䐠Oxidation ditch (OD) 䐡Membrane bioreactors (MBR)Fluidized-bed䐢Carrier-added activated
sludge processPacked-bed
䐣Trickling filter process 䐥Rotating biological contactor (RBC)2. Biological Wastewater Treatment
-Anaerobic & Facultative Treatment-Oxygen
DemandHabitationPopular NamesEffective MicroorganismsAnaerobic
Suspended
growth 䐦CompletelymixedAcidogenesis
Clostridium
Bacillus, ...
Methanogenesis
Methanobacterium
Methanosarcina, ...
䐧Anaerobic contactFixed-bed䐨Up-flow packed beds
Fluidized bed
䐩Fluidized and expanded beds (AFBR) 䐪Up-flow AnaerobicSludge Blanket (UASB)
Stabilization
pondFacultative䐫Facultative typePhotoautotrophs
Chlorobium, Algae,etc.
Heterotrophs
Pseudomonas, etc.
2. Biological Wastewater Treatment
-Advanced Treatment- Removal ObjectPopular NameEffective MicroorganismsBiological Nitrogen
Removal
䐬WuhrmannprocessAmmonia-oxidizing bacteriaNitrosomonas
Nitrite-oxidizing bacteria
Nitrobacter
Denitrifying bacteria
Pseudomonas, Microccus,
Paracoccus,Alcaligenes,etc.
䐭Nitrification liquid circulation process 䠆䠅AnammoxprocessAmmonia-oxidizing bacteriaAnammoxbacteria*)
Biological Phosphorus
Removal
䐮Anaerobic-aerobicASPDephosphorization bacteria*)
Biological Nitrogen
and PhosphorousRemoval
䐯Anaerobic-anoxic ASPAmmonia-oxidizing bacteria
Nitrite-oxidizing bacteria
Denitrifying bacteria
Dephosphorization bacteria*)
Note*): Ecology has not been fully elucidated
2-1. Aerobic Treatment
䐟Conventional Activated Sludge Process(CASP)Aeration TankReturn SludgeEffluent
( ) : population in activated sludge2-1. Aerobic Treatment
䐟Food Chain in Activated Sludge2-1. Aerobic Treatment
䐟The greatest feature of CASP •CASP greatest feature is that the bacteria (activated sludge) grown by blowing air into sewage in the aeration tank is transported to the sedimentation tank, and then separated into solid and liquid, and again returned to the aeration tank. •Accordingly, it is possible to manage separately the time required for the bacteria growth (generation time) and hydraulic residence time in the aeration tank (water treatment time).2-1. Aerobic Treatment
䐟Advantages and Disadvantages of CASPAdvantages
䞉High reduction of BOD and pathogens (up to 99%) at after secondary treatment 䞉Can be operated at a range of organic and hydraulic loading rates 䞉High effluent quality 䞉Little land required compared to extensive natural system (e.g. Stabilization ponds)Disadvantages
䞉High energy consumption, a constant source of electricity is required. 䞉High capital and operating costs 䞉Requires operation and maintenance by skilled personnel 䞉Prone to complicated chemical and microbiological problems 䞉Sludge require further treatment and appropriate discharge Resource : Sustainable Sanitation and Water Management (SSWM)2-1. Aerobic Treatment
䐟Typical Process Operating Factors (ASP)Process
Modification
Volumetric
䠄kg BOD5/ੑ䞉d䠅 MLSS 䠄mg/l䠅 F/M 䠄kg BOD5/kgMLVSS䞉d䠅
BOD Removal
Efficiency
SRT 䠄d䠅Conventional0.61,000~3,0000.2~0.5954~10
Contact
Stabilization1.0A0.2~0.5904~15
Modified
Aeration1.5~6300~6000.5~3.560~85B0.8~4
Extended
Aeration0.33000~50000.05~0.285~95B>14
Source : Bruce E. Rittmann, Perry L. McCarty : Environmental Biotechnology: Principles and Applications
A : Contact Tankї1,000~3,000mg/l ;Stabilization Tankї5,000~10,000mg/l B : Higher efficiency is based upon soluble effluent BOD5.2-1. Aerobic Treatment
䐠Oxidation Ditch(OD)Characteristic of Oxidation Ditch Process
1.Operation management is easy.
2.Even if there is an hourly fluctuation of inflow load and a decrease of water
temperature, this process can complete stable organic removal.3.The amount of excess sludge generated of this treatment method is less than
that of the conventional activated sludge process.2-1. Aerobic Treatment
䐡Membrane Bioreactors䠄MBR䠅Characteristic of Membrane Bioreactors
1.Process function is not affected by the change of the sludge settling
properties.2.Since we can keep the MLSS concentration high in the reaction tank , it
is possible to make the facility compact.3.Treated water is clarified, it is also possible to apply directly to recycling.
Resource : Guidelines for membrane treatment technology introduction into the sewer [1st edition],May 2009