[PDF] [PDF] How to Make a Serial Dilution - ASSET - Cornell University

Pipette 20 μl of the D1 solution into your D2 tube Gently press the plunger on the pipette up and down to mix Now you have a dilution of 1:100 (10-2) 



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[PDF] Serial Dilutions

What is the total dilution that we need to make? Stock concentration: 10 mg/mL= 10-2 g/mL Final concentration: 1 ng/mL = 10-9 g 



[PDF] How to Make a Serial Dilution - ASSET - Cornell University

Pipette 20 μl of the D1 solution into your D2 tube Gently press the plunger on the pipette up and down to mix Now you have a dilution of 1:100 (10-2) 



[PDF] Serial Dilutions

Traditionally serial dilutions were done with 1mL pipettes and 99mL sterile dilution bottles because they were If you start out with a broth culture and do a 10 -6 dilution Using a new sterile 1mL pipette, tranfer 1mL from the 1:10,000 ( 10 -4 ) 



[PDF] PREPARING SOLUTIONS AND MAKING DILUTIONS

milligram = mg = 1/1000 of a g or 10-3 g gram = g However, if you are asked to make a 1:3 dilution of acetic acid in ethanol, you would mix A serial dilution is simply a series of simple dilutions which amplifies the dilution factor quickly



[PDF] Math and Dilutions

Microbiology is full of Math and Dilution Terminology Luckily, once 10-1=1/10= 0 1 (Ten to the minus one or one tenth) make a serial dilution of 10-2 overall



[PDF] Dilutions Occasionally a solution is too concentrated to be used as it

1 Make a 1 to 10 dilution of serum in saline 2 Make a 1 in 10 dilution of serum in saline 3 Make a This type of dilution series is referred to as a serial dilution



[PDF] SERIAL DILUTIONS – TUBE METHOD Principle Serial dilution is a

A small amount of serum or solute can be serially diluted by transferring aliquots to diluent One of the most common series doubles the dilution factor with each 



[PDF] Laboratory Math II: Solutions and Dilutions - NIH Office of Intramural

looking at how to make a solution from a solid substance or “solute” being This means that you add 10 milliliters of the 5M NaCl stock solution to a While serial dilutions are a great way to save on both reagents and space in the lab,



[PDF] Making Dilutions

You could make a liter of 10mM NaCl with 0 29 g, because Making Dilutions 3 Figure 1 A serial dilution A small volume (vt) of solution is transferred to a 

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