[PDF] [PDF] Cell Biology

[PDF] Hypo, Hyper, Iso Notes

Hypotonic Solution • Isotonic Solution Solution (HYPO = the cell is going to BLOW) a What type of solution are these cells in? Hypertonic Hypotonic Isotonic

[PDF] Osmosis & Diffusion, What you need to know:

5 Correctly apply the comparative terms of: hypertonic, isotonic, hypotonic 6 Discuss the effects on plant and animals cells of being in each of these solutions

Action of Hypotonic, Isotonic and Hypertonic Solutions upon the

To tube 4, four mils of a hypertonic solution (2 55 per cent) were added which caused a rapid and greater destruction by crenation of the red cells than the 1 70 per 

[PDF] Cell Biology

Cell Biology – Module 2 Osmosis – Hypotonic, Isotonic and Hypertonic solutions Before completing this worksheet, watch the following clips for background 

Hypertonic and Hypotonic Solutions

escape), this solution caused an initiation of cell division and also an increase in the viscosity of the protoplasm of most of the eggs Addition of NaOH in 

[PDF] Osmosis under the microscope - GTAC

When cells are bathed in a solution where the solute concentration is lower than inside the cell cytoplasm (a hypotonic solution) the cell will gain water In this 

[PDF] PLANT CELLS & OSMOSIS - Esalq

Demonstrate cell reactions to hypotonic and hypertonic solutions 2 Define hypotonic and Freshwater is hypotonic to Elodea A hypotonic solution is a solution

[PDF] Study on the Effect of Different Hypotonic Solutions on

13 fév 2013 · Like osmotic pressure, tonicity is influenced only by solutes [1] Here in karyotyping we use hypotonic solution for the rupture of cells The extra 

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Cell Biology ʹ Module 2

Osmosis ʹ Hypotonic, Isotonic and Hypertonic solutions Before completing this worksheet, watch the following clips for background information. Hypertonic, Hypotonic and Isotonic solutions by BOGOBiology.

Diffusion and Osmosis by Science Sauce.

Activity 1 ʹUse the word bank to complete the cloze exercise

Osmosis is defined as the movement of __________ across a semi-permeable membrane (like a cell membrane).

Like all forms of diffusion, osmosis follows a concentration gradient, where water will move from ________ of fairly

high water content (eg pure water) to areas of fairly low water content (eg concentrated solutions).

Cells contain solutions of ______________ substances. If a cell were to be placed into pure water, the water would

move across the membrane _________ the cell. This inrush of water would eventually cause the cell to _________ -

a process called lysis. In this case: the water is said to be hypotonic.

If a cell was placed in a concentrated solution of salt or sugar, the relative levels of water would be higher inside the

cell than out, and water would leave ______ cell, causing it to shrivel. Salt and sugar can cross the cell membrane,

but they need to do this via specialized channels and therefore their movement is ___________ than that of water

which diffuses quickly across the membrane. In this case: the solution is said to be ______________.

placed in isotonic solutions do not lyse or ___________. For this reason, isotonic solutions are used to manipulate

and wash cells.

Word Bank

Dissolved, slower, areas, shrivel, the, water, burst, hypertonic, into

Activity 2

Construct a labelled diagram to express what happens when a cell undergoes lysis.

The following investigation uses mammalian cells grown in culture. In the previous module, you learnt about the

origin of HeLa cells. SPARQ-ed teachers prepare the HeLa cells by growing them in media in a 6 well plate. Each well

in the plate can be used as a separate test chamber. HeLa cells stick to the walls of the chamber, allowing the

removal of the growth medium. We can then add other solutions and observe how the cells react under the

microscope.

SPARQ-ed teachers prepared a 15g/L stock solution of sodium chloride (salt). The stock solution needs to be diluted

to provide a range of solutions to test.

Activity 3

Calculate the volumes of stock solution and water to make each solution. 0g/L and 15g/L are done as an example for

you.

Concentration 0g/L 3g/L 6g/L 9g/L 12g/L 15g/L

Dilution from 15g/L 0 in 15 15 in 15

Volume of stock

solution needed

0mL 15mL

Volume of water

needed

15mL 0mL

Total Volume 15mL 15mL

Before the experiment took place. The cells, in their pink growing medium, were placed on the Floid Microscope

and observed.

Activity 4

Predict what will happen to the cells at 3 different salt concentrations (eg 0g/L, 9g/L and 15g/L)

Cognitive Verbs and Sentence starters

Cognitive Verb QCAA Definition Sentence Starters

Use operate or put into effect; apply knowledge or rules to put theory into practice n/a Construct create or put together (e.g. an argument) by arranging ideas or items; display information in a diagrammatic or logical form; make; build n/a Calculate determine or find (e.g. a number, answer) by using mathematical processes; obtain a numerical answer showing the relevant stages in the working; ascertain/determine from given facts, figures or information n/a Predict give an expected result of an upcoming action or event; suggest what may happen based on available informationquotesdbs_dbs17.pdfusesText_23