Spontaneous excision of BAC vector sequences from bacmid PDF Baculovirus-DNA-Instability-Reference-2.pdf

2003 · Cité 109 fois — from bacmid-derived baculovirus expression vectors upon passage in insect cells Gorben P Pijlman,3

3 Transfect the recombinant bacmid DNA into the insect cell line of choice to generate a

Bac-to-Bac Baculovirus Expression System - Thermo Fisher

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minipreps (1) Grow a single colony in 3 mL of LB containing 50 μg/mL kanamycin, 7 μg/mL

Cité 2 fois — Recombinant bacmid DNA can be rapidly isolated from small scale cultures and then

2003 · Cité 109 fois — from bacmid-derived baculovirus expression vectors upon passage in insect cells Gorben P Pijlman,3

preparation and infection of insect cells - 29 - E Appendix - 31 - E 1 Verifying bacmid integrity

Baculovirus, Bac-to-Bac, bacmid, insect cell, protein expression Introduction The most

rain DH10Bac contained the baculovirus modified DNA (Bacmid) with a mini-attTn7 target site and