[PDF] Visium Spatial Protocols – Tissue Preparation Guide PDF VisiumSpatialProtocols-TissuePreparationGuide.pdf

Frozen Tissue Embedding Prior to cryosectioning, frozen tissue samples are embedded in OCT Unlike paraffin or resin- based embedding techniques, OCT does

tissues in 30 sucrose, snap-frozen tissues, or OCT embedded frozen blocks sucrose in 1×PBS or frozen embed your tissue samples in OCT compound and

[PDF] Cryosectioning Techniques The Basics: Tissue Freezing

Tissue is in OCT and may be frozen using dry ice or the flash frozen method 3 Enzyme study tissue freezing – Often used for fresh muscle tissue A fresh frozen

[PDF] C2 Procedure for freezing tissue in OCT for cryocutting

Reagents: Liquid nitrogen 2' Methybutane (Isopentane) OCT embedding compound Plastic embedding mold Forceps Metal cup Container for liquid nitrogen

[PDF] OCT user manualRev 9 - Labset

Tissue-Plus® O C T Compound User Manual Optimal Cutting Temperature Embedding Medium for Frozen Tissue Specimens

[PDF] Freezing tissues for histology

Embedding protocol for Frozen Samples: 1 Set up equipment for OCT (Optical Cutting Temperature) (Fisher Scientific, Cat#4583) or NEG50TM (Richard-

[PDF] OCT Embedding Medium IFU EN - Thermo Scientific

The Thermo Scientific™ OCT is an embedding medium used to aid sectioning of frozen tissue samples in a cryostat Warnings and Precautions For in vitro

[PDF] OCT Embedding Matrix for Frozen Sections - CellPath

OCT cryo microtomy embedding medium is designed for use at -20°C to -30°C The medium freezes rapidly with minimal damage from crystal formation