Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles ethanol, 70 ethanol, 2 X 100 ethanol, methylbenzoate (MB), 50/50 v/v MB and medium (Tissue-Tek O C T compound) upside down in a 4°C fridge in order to cool it
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[PDF] IHC: Frozen section vs paraffin section Downloadable - Proteintech
Cover the entire tissue block with cryo-embedding media (e g OCT) f Slowly place the base mold containing the tissue block into liquid nitrogen until the entire
[PDF] 0803006 f20 Sectionnement des tissus enrobés de paraffine et d
1 jui 2012 · formaldéhyde et enrobés de paraffine (FFPE-«Formaldehyde fixed and paraffin embedded» et les tissus congelés dans l'OCT peuvent être
[PDF] Comparison of Immunohistochemical Staining of Signaling Markers
OCT or fixed for 24 hours in 10 neutral buffered forma- lin, then processed and embedded in paraffin per standard methods Frozen sections were cut at 7-8
[PDF] Step by Step instruction for frozen sample preparation for histology
sucrose in 1×PBS or frozen embed your tissue samples in OCT compound and submit the sample for frozen section, the thickness is not as critical as paraffin
[PDF] Paraffin-Embedded and Frozen Sections of Drosophila - CORE
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles ethanol, 70 ethanol, 2 X 100 ethanol, methylbenzoate (MB), 50/50 v/v MB and medium (Tissue-Tek O C T compound) upside down in a 4°C fridge in order to cool it
[PDF] OCT user manualRev 9 - Labset
Never freeze and thaw frozen tissue because ice crystal artifacts will occur Remaining frozen tissues may be paraffin embedded through a special processing
[PDF] Immunohistochemistry (IHC) Handbook - Novus Biologicals
Tissues are sectioned from fixed embedded (e g IHC-Paraffin or plastic) or Wait until tissue in OCT is completely frozen (OCT turns from clear to opaque white solid of the antibody should also be considered: monoclonal vs polyclonal 10
[PDF] A procedure for tissue freezing and processing applicable to both
6 fév 2008 · chemical analysis of tissue sections cut from paraffin blocks For rapid DNA, and protein) of tumor tissues embedded in OCT are also discussed Compared to tissues treated with carbon dioxide gas, the morphology of the
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