HISTOLOGY - ROUTINE AND SPECIAL STAINING

SURGICAL PATHOLOGY - HISTOLOGY Date: STAINING MANUAL - CARBOHYDRATES Page: 1 of 3 PAS - McMANNUS' PERIODIC ACID SCHIFF'S - GLYCOGEN PURPOSE: Glycogen is present in skin, liver, parathyroid glands and skeletal and cardiac muscle The PAS stain is used for demonstration of basement membranes, fungus secreting adenocarcinoma from

PAS (Periodic Acid Schiff) Staining Protocol

PAS (Periodic Acid Schiff) Staining Protocol Used for the detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed paraffin-embedded tissue sections, and may be used for frozen sections as well Solutions and Reagents: 0 5 Periodic Acid Solution

ALCIAN BLUE-PAS (PAB) PURPOSE: Routine stain for GI biopsies

SURGICAL PATHOLOGY - HISTOLOGY Date: STAINING MANUAL - CARBOHYDRATES Page: 1 of 2 ALCIAN BLUE-PAS (PAB) PURPOSE: To differentiate between neutral and acetic mucosubstances Routine stain for G I biopsies CONTROL: Use a mucin control FIXATIVE: 10 formalin or Hollande's fixative TECHNIQUE: Cut paraffin sections 4-5 microns thick

HISTOLOGIC - IHC WORLD, LLC

Schiff (PAS) stain in the hope of attaining a rapid and more fool-proof stain This method is among the most frequently employed techniques in histology labora-tories It is relatively simple to perform and reliable, even in inexperienced hands The PAS stain will reliably demonstrate the polysaccharide-laden wall of most fungal organisms, with the

Which stains Special stains in liver pathology

• PAS-diastase • Reticulin • Copper • Other: elastic, PAS, bile Process Role Principle Iron hematoxylin Nuclear stain Works well in acidic solutions Red dye: Acid fuchsin (Biebrich scarlet) chromotrope 2R Stains cytoplasm, muscle Intermediate molecular weight, stain both collagen and muscle Polyacid (phospho-tungstic acid) Removes red

SPECIAL STAINS IN HISTOLOGY

STAINS COMPONENTS STAIN FUNGI 1 SILVER STAINS (GROCOTT METHENAM INE SILVER NITRATE-GMS) 2 PAS FUNGI (BLACK) MUCIN (TAUPE TO GRAY) TISSUE –GREEN FUNGI CELL WALL - MAGENTA • Seen fairly well in H&E, but are demonstrated well with GMS and PAS (yeasts, hyphae and spores) • Fungal cell walls are rich in polysaccharides which can

Education Guide Special Stains - diyhplus

muscle in the liver, with muscle staining red, collagen blue and fibrin pink; C) This PAS stain is used for the visualization of mucopolysaccharides and glycogen in renal tissue Mayer’s hematoxylin counterstains the nuclei blue