Comparison of the qPCR and the HybriScan Legionella assays vs
Comparison of the qPCR and the HybriScan Legionella assays vs culture for the identification of Legionella sp in non-potable water samples Legionnaire’s disease is a severe form of pneumonia caused by Legionella sp , with the majority of cases due to Legionella pneumophila Outbreaks and sporadic cases of legionellosis within communities have
Comparison ofBlood Culture Methods Recovery Legionella
statistic wasusedforcomparisonbetweenthe meannumber of days to first positive blood culture for each method (2) Descriptive statistics of quantitative colony counts were derived by using an assumption of a Poisson distribution (13) Computer-aided analysis of variance was used for comparison of the mean L pneumophila concentrations
Comparison of conventional culture and real-time quantitative
Legionella levels in water are assessed routinely by culture on a selective medium, but its slow growth is a serious drawback, given that at least 10 days are required to obtain results In an attempt to provide a simple screening method for Legionella pneumophila in water systems samples a real time PCR assay using SYBR Green was developed
The determination of Legionella bacteria in waters and other
Growth does not occur in the absence of L-cysteine, except that Legionella oakridgensis,and Legionella spiritensis (which require L-cysteine and iron(III) on primary isolation) can grow weakly in the absence of L-cysteine thereafter Accordingly, careful comparison needs to be made of the differences in growth between supplemented and un-
Certificate of Analysis Legionella pneumophila CRM12821M
Certificate of Analysis Legionella pneumophila CRM12821M This certificate should not be parted from the PHE certified reference material with batch number: 1026-15 Uncertified data and guidance for use can be found at the end of this certificate 1 Public Health England Reference Material (RM) Manufacturer
Comparison of disinfectants for biofilm, protozoa and
analysis) Glass beads for biofilm analysis were soaked in sterile water, sonicated for 2min, and the resulting suspen-sion was analysed for sessile Legionella and amoebae (300 and 400 beads, respectively) Legionella were analysed according to ISO 11731 culture plate assay (ISO 1998) (culture on buffered charcoal-yeast extract with 0 1 a-
ScanVIT-Legionella
of Legionella Despite this long analysis time, the conventional method is often not specific enough With ScanVIT-Legionella harmful Legionella bacteria can be identified and quantified precisely in water samples within only 3 days The detection of Legionella takes 75 hours from sampling until the quantitative results are available
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