TITRE IX TOPOGRAPHIE – OBSERVATION
– TTA 501 : Règlement de topographie – TTA 502 : Manuel de topographie Une très bonne connaissance des sections I et II de ce titre est fondamentale : un bon usage de la carte et de la bous-sole conditionne souvent l'accomplissement des missions de combat Aussi, est-il souhaitable que l'étude de ces deux sec-
TITRE IX TOPOGRAPHIE - OBSERVATION - ÈreWerra
TTA 150 TITRE IX TOPOGRAPHIE - OBSERVATION Expert de domaine : EAA Edition 2008 ANNEXE A - S’ORIENTER A 1 1 DÉFINITION S'orienter, c'est déterminer l'endroit où
TITRE I CONNAISSANCES MILITAIRES GÉNÉRALES
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Chemical Equilibrium and Synergism for Solvent Extraction of
2) for Li-TTA/2TOPO was 150 times higher than Li-TTA/TOPO The distribution coefficient of Li-TTA/2TOPO into m-xylene was 9 12 and the logarithmic extraction constant (log K ex) was 6 76 Trace lithium of sub-ppm level in seawater samples could be determined under modified
on and Analytical Applications of Synergistic Solvent
extraction using TTA and TOPO with an aliphatic hydrocar bon such as n-hexane than with an aromatic hydrocarbon solvent And the complex was nearly non-extractable in a polar solvent such as chloroform This proves that Li-TTA- TOPO adduct was bulky and non-polar But, MIBK is a sol vent mainly used in solvent sublation because of its high
Hitachi Fluorescence Spectrophotometer
Phosphorescence spectrum measurement of Eu (tta)3(TOPO)2 complex Phosphorescence life measurement of Eu(tta)3(TOPO)2 complex Wavelength (nm) Fluorescent intensity EGF Conc WL 340, 510nm, WL 380, 510nm, 0 50 100 150 200 sec 300 200 100 Ca 2+ (nM)
Decreasing luminescence lifetime of evaporating
oacetone-trioctylphosphine oxide (Eu-TTA-TOPO), in a heptane solution (99 Reagentplus, Sigma-Aldrich) The Eu-TTA-TOPO is a phosphorescent lanthanide supramole-cule with a phosphorescence lifetime in the order of millisec-onds at a dominant wavelength of k 614nm It is one of the lanthanide complexes useful for flow tracking and evapo-
Luminescent chemiluminescence - PNAS
IVEu(TTA)3Phen 0 37t 1 8 0 46 (100) 0 6 x1/2 isthetimefor 50 o decreasein signalduringmultiphasicdecay Numbersin parentheses indicate the concentration ofEuin mM
Simultaneous topographic and chemical patterning via
(Eu(TTA) 3(H 2O) 3) purchased from Acros were mixed with poly-(vinylpyrrolidinone) (PVP, Aldrich, M w ¼ 120 kg mol 1,glass transition temperature T g ¼ 127 C) in ethanol and polystyrene (PS, Aldrich, M w ¼ 125–250 kg mol 1, T g ¼ 105 C) in toluene, respectively The nal concentration was 100 mg L 1:40mgL1
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Hitachi Fluorescence Spectrophotometer
12 Designed to Meet Your Needs for High-Quality Analytical Instrumentation.Hitachi's Superior Fluorescence TechnologyHas Created a New Generation ofFluorescence Spectrophotometers.
High S/N Ratio, Ultra-Fast Scanning, Compact Design,Multiple Accessories High sensitivity measurement (S/N 800 RMS)(equivalent to S/N 250 peak to peak)60,000 nm/min ultra-high scanning speed, ideal for 3-D measurement.
Compact design (approx. 2/3 the size of the F-4500) A wide range of accessories accommodating various applications P.3~4 P.5~6 P.7~8P.9~10
Outstanding
Performance
supported by SuperbManufacturing
Technologies
Sensitivity, Scan Speed,
3-D Measurement,
and more....Extensive
Application
Capabilities
Industrial Materials,
Pharmaceutical, and
Biotechnology.
Easy-to-Use
Software
A Wide Range of
Accessories
34Technologies Supporting Hitachi Fluorescence Spectrophotometers
F-7000 Performance Supported by Technology
Precision Machining Technology
resulting in bright optics.Advanced Electric Circuit Technology for high-speed processing.Controlled System Technology ensures high accuracy. ?Stigmatic concave diffraction grating, mechani- cally ruled, resulting in a very bright monochro- mator of F-number 2.2.Ruling engine.
A dividing engine for ruling diffraction gratings, invented in 1880s by Henry Augustus Rowland of Johns Hopkins University. Compared to a holographic grating, mechanically ruled gratings have the following advantages: (1) Mirror-finished groove surface results in high diffraction efficiency. (2) Groove spacing required for aberration correction can be adjusted, making it possible to have a greater correction effect. These characteristics of mechanically ruled gratings work well to create an excellent monochromator. ?Detection limit of fluorescein Due to enhanced sensitivity, the F-7000 Fluorescence Spectrophotometer can detect fluorescein concentrations one digit lower than its predecessor, the Model F-4500. The improved S/N ratio provides greater capabilities for trace-sample measurements. ?Example of reaction tracing with a spectrum The fast-speed scanning enables users to carry out measurements that have been difficult with conventional instruments. In this example, an isomerization process of coumarin in kerosene was traced by spectrum measure- ment at 2-second intervals and displayed as a 3-D time- scan spectrum. This is a new function in the F-7000. Previously, a quick reaction which occurs within 1 minute could be measured only by using the fixed-wavelength method. The F-7000 is capable of following such a quick reaction because of its fast scanning, measuring the entire wavelength range within 1 second. ?Data of fluorescent marker penA 3-dimensional fluores-
cence spectrum can clear- ly distinguish slight differ- ences that a 2-dimenstion- al spectrum cannot detect.Measurements can now
be carried out with higher accuracy than before. ?High S/N: 800 (RMS), 250 (Peak to Peak) ?3-D Measurement ?High-Speed Scan at 60,000nm/min ?Calibration curve and spectrum overlay of fluores- cein The automatic gain change-over function, a technique unique to Hitachi fluorescence spectrophotometers, has made it possible to generate calibration curves using up to 6-digit concentration values. An unknown sample can be quantitatively analyzed without additional sample preparation. ?Automatic pre-scan function optimized for unknown sam- ple measurement ?Ratio photometry (0 point correction) ensuring stable measurements ?High-resolution multi-stage slit with a resolution as small as 1nm ?Shutter control for minimizing sample deterioration ?Measures up to 6-digit concentration values ?Other functionsDiffraction grating
1.2×10
- 10 mol/LWavelength
3-dimensional spectrum of
fluorescent marker pen(green ×red)3-dimensional spectrum of fluorescent marker pen (green)2-dimensional spectrum (excitation wavelength 460nm)
1×10
- 12 mol/LFluorescent intensity
3-dimensional time scan spectrum of coumarin for environmental applications
Concentration (mol/L)
Fluorescent intensity
56Application Capabilities Unique to Hitachi
Industrial Material Field
?Organic EL material In this example, the F-7000 was used to analyze the lumi- nescent characteristic of trisaluminum complex powder used as a luminescent material for organic EL display. A solid sample holder, its powder cell, the photomultiplier R928F, and the filter set were used.Scan speed: 12,000nm/min
Excitation slit: 5.0nm
Emission slit: 5.0nm
Photomultiplier voltage: 400V
Response: Automatic
Spectrum correction: Activated
Beam-cut filter (UV-39) used
Photomultiplier R928F used
?Measurement of fluorescent materials20406080100
460 480 500 520 540
P2×2e-YFP
20460 480 500 520 54040
6080100
Negative control
Biological Field
?FRET (Fluorescence Resonance Energy Transfer) and BRET (Bioluminescence Resonance Energy Transfer) The Model F-7000 can measure the intermolecular activi- ties such as FRET and BRET. Shown below are fluores-cence spectra presenting the interactions between the sub-unit proteins of an ATP-active purine receptor.
Data provided by Mr. Takaaki Koshimizu, Kyoto University Graduate School of Pharmaceutical Sciences - Genomic Drug Discovery Science. ?Measurement of intermolecular actions ?Measurement of calcium in cellPharmaceutical Field
?Rare earth element complex (Eu chelate) The example below shows the phosphorescence spectrum and lifetime measurement of the Eu(tta)3(TOPO)2complex,
a rare earth element.?Ca 2+ concentration in cells With the optional interacellular calcium measurement accessory, the F-7000 can measure fluorescence intensity values at two wavelengths in EGF-injected COS-7 cells (extracted from a monkey's kidney), and calculate the con- centrations of Ca 2+ The sample was a cultivated cell fluorescence-labeled byFura2-AM.
The change in Ca
2+ concentrations in the live cell was also measured. During this analysis, the EGF receptor appeared in the COS-7 as the Ca 2+ level increased due to EGF injec- tion. The Model F-7000 can measure biological samples with higher sensitivity and speed. ?Micro-plate Accessory ?Features ?Automatic measurement using a 96 well microplate is available. ?Used as an autosampler, allows wavelength scan, time scan, and 3-dimensional measurement. ?Can be used in conjunction with the polarization accessory.With the F-7000, the analysis of phosphorescence life of 1 ms order can be performed at room temperature without special accessories. ?Phosphorescence measurementThe acquisition of these data was made possible by the 3-D measure-ment function and high-speed scanning capability of the F-7000.
Phosphorescence spectrum measurement of Eu (tta)
3(TOPO)2complex Phosphorescence life measurement of Eu(tta)3(TOPO)2complex
Wavelength (nm)
Fluorescent intensity
EGFConc. WL 340, 510nm, WL 380, 510nm,
0 50 100 150 200sec300
200100
Ca 2+ (nM)