[PDF] Mapping transcription factor interactome networks using

Implication of Tubby Proteins as Transcription Factors by

Most tubby proteins include NH 2-terminal regions that, in general, are not closely related to one another These NH 2-terminal regions, however, are often similar in cross-species orthologs They comprise about 180 to 280 amino acids in tubby and TULPs 1 through 3 Recent technological developments have opened a new possibility for the application

The Application of SPA Technology to Study Protein: DNA

application note The Application of SPA Technology to Study Protein: DNA Interactions acrylamide gels(2) Thus, the mobility shift assay begins to indicate the potential for a gene to be transcribed by providing an assay for the presence of DNA-binding proteins capable of binding to a promoter Although this is a very simplistic approach compared

Mapping transcription factor interactome networks using

proteins in situ eliminate the cumbersome process of expressing and purifying individual proteins and hence avoids compromising protein functional integrity Additionally, because DNA is spotted instead of proteins, the arrays require no special storage condi-tions and have an extended shelf life of greater than 12 mo

Engineering zinc finger proteins for therapeutic applications

4 Number of proteins Genome-wide comparison of transcriptional activator families in eukaryotes Tupler R, Perini G, Green MR, Nature 409: 832 The C2H2 zinc finger: Mediator of metazoan evolution

G-Quadruplexes: Prediction, Characterization, and Biological

Transcription et traduction : Exercice d’application

Transcription et traduction : Exercice d’application L’ocytocine et la vasopressine sont deux hormones synthétisées par la post-hypophyse des Mammifères La première a comme organe-cible l’utérus et la seconde les artères et les reins Voici une portion des séquences nucléotidiques codants pour ces 2 protéines

PROTEOLYSE - PROTEOSYNTHESE- RENEVOULLEMENT DES PROTEINES

particulièrement dans l'application de la « peine de mort » cellulaire, appelée mort cellulaire programmée : apoptose ücapacité d’hydrolyser des chaînes polypeptidiques au niveau d’un acide aspartique situés dans la partie C-terminale de la protéine substrat ü synthétisées s/f de zymogènes =pro-caspases inactifs

Introduction aux techniques de biologie

Facteur de transcription HIF-1α (Hypoxia Inducible Factor) - Présence O 2 cytoplasmique - Absence O 2 nucléaire transcription de gènes nécessaires à la survie cellulaire Fibroblastes de membrane synoviale humains + mimétique (CoCl 2 150 µM) pendant 24 h Témoin CoCl 2