PULSE-CHASE PRIMER: THE MESELSON–STAHL EXPERIMENT
Mar 03, 2016 · PULSE-CHASE PRIMER: THE MESELSON–STAHL EXPERIMENT This activity can be used in conjunction with the short film The Double Helix After publishing the structure of DNA, Watson and Crick published a second paper that stated their hypothesis of how DNA replicates They predicted that
Pulse-Chase Experiment - Jacobs University Bremen
Pulse-Chase Experiment Objective: To follow a specific cohort of molecules by radiolabeling them with a radioactive amino acid Summary: In a pulse chase experiment, cells are grown in medium and exposed for a short time period to a large amount of radioactive amino acid This is called the pulse, and during this time
The Double Helix TEACHER MATERIALS
• If students are not already familiar with a pulse-chase experiment, explain that it is a two-phase technique used to examine cellular processes that take place over a period of time During the pulse phase of the experiment, cells are exposed to a labeled compound The labeled compound is incorporated into the molecule or pathway being studied
PULSE-CHASE PRIMER: THE MESELSON-STAHL EXPERIMENT
PULSE-CHASE PRIMER: THE MESELSON-STAHL EXPERIMENT INTRODUCTION In the 1950s, James Watson and Francis Crick suggested a mechanism for the replication of DNA, which they called the “Semiconservative Model of Replication ” During replication, each of the two strands of DNA would act as a template for the synthesis of a new strand
ULTIMATE YEAST PULSE-CHASE - Cyr Laboratory
May 11, 2014 · 1 Each pulse-chase will require a 20 ml culture of yeast at an OD 600 of between 0 4 and 0 6 To do this, I usually set up a number of 20 ml cultures the evening before, innoculated at different densities, so that first thing next morning at least one of the cultures will be at an OD 600 of ~0 5 B PULSE-LABELING
Pulse and chase labeling with [35S]-Methionine
- Pulse: Add 0 35 ml of [35S]-Meth working solution Incubate 30 min at 37°C - Chase: Add 0 75 ml of chase medium to the [35S]-Meth solution and remove - Wash once with 1 ml of pre-warmed chase medium - Add complete medium (DMEM + 10 FBS) Treatments, such as Wnt3a or Lactacystin, can also be added at this point
Pulse-Chase Labeling of Yeast with 3H]-Methyl Methionine or
Pulse-Chase Labeling of Yeast with [3H]-Methyl Methionine or [3H]-Uracil • Plan for: 0 5 A600 unit of cells per chase time, 0 5 ml of labeling medium per A600 unit, 25 µCi of radioactive label per A600 unit, 5 µl of volume to resuspend RNA pellet from 1 A600 unit of cells (enough for 2 gels)
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