PREPARATIVE DENSITY GRADIENT CENTRIFUGATIONS

Chapter 3 Centrifugation Biochemistry and Molecular Biology (BMB) 3 1 Introduction 3 2 Basic Principle of sedimentation 3 3 Types, care and safety of centrifuges 3 4 Preparative centrifugation 3 5 Analytical centrifugation Analytical Biochemistry (AB) 3 4 3 Ultracentrifugation Koolman, Color Atlas of Biochemistry, 2nd edition

Ultracentrifugation: Probably the best downstream processing

Centrifugation KII ULTRACENTRIFUGATION Start volume 88L Rotor Volume Waste Volume 88L Gradient 60 Sucrose Flow Rate 15L/h Speed: 35 000 rpm Time 1 runs of 7h Gradient collected : Final Volume 600ml per run Concentrate 75x Yield 80 16

Analytical Ultracentrifugation in the Study of Protein Self

centrifugation, a 10 to 1000fold concentration range is typically observed, and a size-range of three orders of magnitude in molar mass can be covered in a single experiment Interacting components under study may have sizes ranging from peptides to very large multi-protein complexes In

AN OVERVIEW OF ANALYTICAL ULTRACENTRIFUGATION (AUC)

Analytical ultracentrifugation (AUC) is the most versatile, rigorous and accurate technology available for determining the molecular weight, hydrodynamic and thermodynamic properties of a protein or other macromolecule Although its history dates back nearly a century, AUC is:

Comparison of ultracentrifugation methods for concentration

with other viruses (Peng et al 2006; Gias et al 2008) After ultracentrifugation according to the first protocol, the supernatant was removed and the virus-containing pellet was resuspended by gentle agitation in 1 mL of TNE solution overnight at 4 °C After ultracentrifugation

Analytical Ultracentrifugation In Protein Science

equation (Teller et al 1979; van Holde et al 1998), in which the R o of the sphere is expressed as 1/3 4 3 π υ NA M, to obtain ( ) 1/3 4 3 6 1 π υ πη − υρ = A A sphere N M N M s (3) Substituting the values for all the constants (h for water at 20oC) yields Eq 4, the s value for a