Tips and Tricks for HPLC and UHPLC

HPLC at UAF is an Agilent 1100 Series and is located in downstairs instrument room (Figure 2) Figure 2: Labeled Agilent 1100 Series HPLC at UAF HPLC data is given in the form of a chromatogram, which looks much like data from other instruments (Figure 3)

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

High Performance Liquid Chromatography (HPLC) An analytical separation technique that involves the high-pressure flow of a liquid through a column that contains the stationary phase Stationary phase: Can be a solid (LSC) or a liquid (LLC) A mixture of compounds injected at one end of the column separates as the compounds pass through

High Performance Liquid Chromatography (HPLC)

your background knowledge of HPLC to answer the following questions: Use a separate piece of paper and attach it to your personal pages(or follow instructions from your instructor 1 Which Kool-Aid dye (red or blue) was the most polar? Justify your answer 2 What would have happened if you had used the HPLC solvents in reverse order?

Column separation (liquid-liquid, liquid-solid) used for

High Performance Liquid Chromatography (HPLC) Column separation (liquid-liquid, liquid-solid) used for separating and analyzing compounds based on differences in their interaction with a stationary phase Adsorption, partition, ion exchange, molecular exclusion and affinity

METHOD 8330A NITROAROMATICS AND NITRAMINES BY HIGH

6 1 HPLC system 6 1 1 HPLC -- Equipped with a pump capable of achieving 4000 psi, a 100-µL loop injector and a 254-nm UV detector (Perkin-Elmer Series 3, or equivalent) For the low concentration option, the detector must be capable of maintaining a stable baseline at 0 001 absorbance units full scale 6 1 2 Recommended columns

Dossier La Chromatographie Liquide HPLC

La Chromatographie Liquide HPLC Figure 1 M canisme dÕexclusion st rique Selon le rapport 2 R h /d , il est possible dÕa voir soit une exclusion (A), une p n tra - tion s lective (B) ou une perm ation totale (C) de la macromo - l cule Figure 2 Chromatogramme SEC dÕun chantillon de polym re