Purification and biochemical characterization of a novel

1 Laboratoire d’Ingénierie des Protéines & des Mole´cules Bioactives, Institut National des Sciences Applique´es et Technologie, Université de Carthage, BP 676, 1080 Tunis Cedex,Tunisia 2 Laboratoire de Biochimie, Environnement & Agroalimentaire URAC 36, Universite´ Hassan II Mohammedia-Casablanca, BP 146, 20650 Mohammedia, Morocco

Pichia Fermentation Process Guidelines Overview

Version B 053002 Pichia Fermentation Process Guidelines Overview Introduction Pichia pastoris, like Saccharomyces cerevisiae, is particularly well-suited for fermentative growth

Climate risk assessment and management in agriculture

71 Climate risk assessment and management in agriculture Ramasamy Selvaraju Climate, Energy and Tenure Division, FAO, Rome INTRODUCTION Climate risk in agriculture represents the probability of a defined hydro-meteorological

FAPBM Reniala FEV/FEB/FEB 2021

précipitation, l’Association Tsimoka mène des investigations depuis l’année 2017 pour la recherche de stratégies efficientes Une des expérimentations mise en œuvre en 2021 est l’utilisation des nouveaux dispositifs (cocoon et waterbox) Merci à Missouri Botanical Garden (MBG) Shared Resources Joint

Contamination des eaux souterraines par les métaux lourds d

considérée comme sèche,avec 207 4 mm de précipitation niveaux p2 et p3 peuvent être considérés comme proté-gés car se trouvant à des profondeurs importantes,il n’en

Le deuil des chiens

tations des mendiants, des cris des gamins, des jérémiades des vieillards Chama passa ses mains sur son visage avant de poursuivre son discours Sa voix brisée par l’émotion et la fatigue résonnait comme des coups de gong dans ma poi-trine J’avais les yeux baissés, le regard rivé sur le pied du lit en fer forgé

J. Mater. Environ. Sci. 5 (5) (2014) 1490-1499 Abidi et al.

ISSN : 2028-2508

CODEN: JMESCN

1490
Purification and biochemical characterization of a novel alkaline protease from Aspergillus niger. Use in Antioxidant peptides production Ferid Abidi1* Neyssene Aissaoui1, Said Lazar2*, Mohamed Nejib Marzouki1

1 & des Mole´cules Bioactives, Institut National des Sciences Applique´es et

Technologie, Université de Carthage, BP 676, 1080 Tunis Cedex,Tunisia

2 Laboratoire de Biochimie, Environnement & Agroalimentaire URAC 36, Universite´ Hassan II Mohammedia-Casablanca,

BP 146, 20650 Mohammedia, Morocco.

Received 9 March 2014; Revised 10 June 2014; Accepted 16 June 2014. *Corresponding authors: feridinsat@yahoo.fr/lazar_said@yahoo.fr; fax: +212 5 23 31 53 53.

Abstract

This work reports the production of a novel alkaline protease from the fungus Aspergillus niger. The protease was purified

from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and

DEAE-sepharose ion exchange chromatography with a 13.9-fold increase in specific activity. The molecular weight of the

enzyme was estimated to be 32 kDa on SDS-PAGE. The optimum pH and temperature were respectively, 9.0 and 50 °C. The

enzyme stability was investigated over broad range of pH, temperature. The protease maintained considerable activity at the

range of 3060 °C and pH 710. The purified Aspergillus niger Protease (Prot-Asp) was used for the production of bioactive

peptides. Grey mullet by products were hydrolyzed with purified protease in order to obtain peptides with biological

activities. Interestingly, the hydrolysate (GMH) revealed the presence of antioxidant peptides. Keywords: Protease alkaline; Aspergillus clavatus; protein hydrolysates; antioxidant activity.

Introduction

Proteases constitute a large group of hydrolytic enzymes that catalyze protein hydrolysis and degrade them into

small peptides and amino acids. The relevance of this group of enzymes, rich in structural diversity and

mechanisms of action is reflected in the importance of their applications in industrial processes [1].

Therefore, the industrial demand for proteolytic enzymes, with appropriate specificity and stability to pH,

temperature and chemical agents, continues to motivate the search for new sources [1]. Thermoalkaline proteases

are the most commonly used of the alkaline proteases because they can function at a pH range of 7.012.0 and a

temperature range of 35quotesdbs_dbs15.pdfusesText_21

[PDF] Purification and biochemical characterization of a novel