pKa values relevant to CEM 850 - Michigan State University

The PH domain of PKB (reproduced with kind permission from Thomas et al [2002]) and catalytic and regulatory domain of PKB (reproduced from Yang et al [2002a]) are shown The regions that are important for the interaction of several proteins are indicated Proteins requiring the complete PH domain (TCL1 and IMPDH) are also listed

pH DES SOLUTIONS AQUEUSES I Généralités

Les équations sont étalies à partir des équations du as d’un mono -acide faible en remplaçant pH par pOH et Ka par Kb avec : pH + pOH = pKe pKa + pKb = pKe 1 Cas d’une solution onentrée d’une ase failement dissoié e (α< 0 05) ????=√???? /????0 et [H 3 O+]

Expression and puriWcation of active PKB kinase from

To produce active PKB kinase from Escherichia coli, we constructed a derivative of PKB lacking the PH domain and mutated to glutamate at residues S124, T450 and the activating residue S473 (PH-PKB-EEE) PH-PKB-EEE was expressed in E coli together with PDK1, the kinase responsible for phosphorylating PKB at T308, which was expressed as a GST

Targeting PKB to GLUT4 vesicles

inactive PKB mutant (Cong et al , 1997), in which the PDK1 and PDK2 phosphorylation sites were additionally mutated [PKB-AAA (Wang et al , 1999)], and inhibitory anti-PKB antibodies (Hill et al , 1999) were found to block insulin-stimulated glucose uptake This is consistent with a role for PKB in mediating insulin-stimulated GLUT4 translocation

pH et pKa - Zysman-Colman

1 1 pH et pKa 2 Pourquoi est-ce utile? Beaucoup de réactions se font en condi tions acides ou basiques, et le chimiste doit être en mesure de pouvoir choisir le “bon acide” ou la “bonne

Molecular Cell, Vol 7, 937–947, May, 2001, Copyright 2001 by

Control of Cell Polarity and Chemotaxis by Akt/PKB and PI3 Kinase through the Regulation of PAKa Tilton et al , 1997) Chemoattractant stimulation of neu-trophils and Dictyostelium cells results in a transient localization of a GFP fusion of the PH domains from Chang Y Chung, Gary Potikyan, and Richard A Firtel1

Buffer pKa and pH Range Values - UNL Animal Science

Buffer pKa and pH Range Values Continued For preparation of Buffers in the pH Buffers pKa Range Succinic Acid – C 4 H 6 O 4 4 2, 5 6 3 2-6 6