TR00064 Introduction
Most sources report extinction coefficients for proteins measured at or near a wavelength of 280nm in phosphate or other physiologic buffer Molar Extinction Coefficients vs Absorbances for 1 Solutions Application of a molar extinction coefficient in the calculation yields an expression of concentration in terms of molarity: A / ε molar
ISO 9001 ISO 14001 ISO 13485 Registered Quality Environmental
and using a molar extinction coefficient of 18,000 M-1 cm-1 (16,000 M-1cm- 1 for 0 5 M EDTA) (1, 3) One unit of the protein phosphatase activity is defined as the amount of enzyme that hydrolyzes 1 nanomole of PNPP in one minute at 30°C in a total reaction volume of 50 μl under standard reaction condition
-N A D P H - OYC Americas
6 2×103＝Molar extinction coefficient of NADPH at 340 nm（L･mol－1･cm－1） d＝Light path length（1 cm） v＝Sample volume（0 5 mL） s＝Sample concentration（0 6 mg/mL） S＝Na（ ） W＝Water Content（ ） Keep tightly stoppered in the dark below 5℃ Moisture will accelerate the purity reduction
b-D-galactopyranoside N1127 Product Description Preparation
nm 2 The molar extinction coefficient is 2 13 x 104 at pH 10 2 3 We have used absorbance readings at 410 nm in the determination of the activity of β-galactosidase using this product as the substrate The K m for β-galactosidase (from E coli) is 9 5 x 10-4 M in Tris buffer, pH 7 6, at 20 °C 4 The V max for β-galactosidase for this substrate is
Calculation of the thickness and optical constants of lead
transparent substrates The film has thickness d and complex refractive index n c =n-ik, where n is the refractive index and k the extinction coefficient The substrate thickness is several orders of magnitude larger than d, and its refractive index is symbolised by s which the dispersion is given by the following relationship [22]: c b s a 2
Myoglobin (M0630) - Product Information Sheet
Extinction coefficient:2 EmM = 12 92 (555 nm) pI:3 7 3 (major component) and 6 8 (minor component) Myoglobin from horse skeletal muscle is a single chain heme protein containing 153 amino acid residues It posesses no disulfide bridges or free -SH groups Myoglobin contains 8 variously sized right-handed
Mesure de l’activité enzymatique
Il est difficile de mesurer la quantité d’enzyme en unités de masse ou de concentration molaire (quantité trop faible et problème de purification); l’activité enzymatique est défini en terme de vitesse de réaction (la V étant directement proportionnelle à la quantité d’enzyme) 2
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