Genetically Engineered Grapevines Carrying GFLV Coat Protein
Grapevines Carrying GFLV Coat Protein and Antifreeze Genes Institute of Genetic Engineering 2232 Kostinbrod-2, Bulgaria Received: December 20, 2000 ACKNOWLEDGEMENTS We thank Dr C Kontz and Dr R Golles for kindly providing the anti-freeze constructs and CP GFLV construct, respectively Georgy P GUTORANOV Ivan J TSVETKOV Violeta M COLOVA
Breeding Rootstocks for California Vineyards
Rootstock Development • 37 did not allow reproduction (egg mass formation evaluated with staining) and were advanced to testing against: – Two strains of RKN selected from Harmony rootstock – HarmA (M arenaria), HarmC (M incognita), and Xiphinema index (the dagger nematode vector of GFLV)
Metagenomic‐based impact study of transgenic grapevine
virus (GFLV) strain F13 (F13-cp) and the neomycin phosphotransferase (nptII) gene, one of the most widely used selectable marker genes in plant genetic engineering (Jelenic, 2003; Turrini et al , 2015)
In Vitro Cell Dev Biol-Plant 35:226-231, May-June 1999 C
GRAPE ROOTSTOCK TRANSFORMATION 227 Genesa Constructsb 1) LR3 43K double 35S AIMV GLRaV3-43K NOS 2) LR3cpST NPI 2enh 35S AIMV GLRaV3-CP 35S 3) LR3cpAS NPTII 2enh 35S AIMVI GLRaV3-CP 135S 4) FLcpST enh 35S AIMV GFLV-CP 35S 5) FLcpAS NPTII 2enh I35S AIMVI GFLV-CP 35S 6) FLcp+GUS NPTII 355S CMV GFLV-CP 35S 7) virE2 delB NPTII double 35S AIMV virE2
Interactive Technology Assessment’’ and Beyond: the Field
where genetic engineering is one of the few means available to solve a major agronomic problem or to study funda-mental mechanisms of plant biology, as a first step For instance, Grapevine fanleaf virus (GFLV), which is transmitted by soil nematodes, is the cause of an often lethal disease of grapevines with worldwide distribution
virE2 AGROBACTERIUM TUMEFACIENS - Springer
is the GFLV sense translatable coat protein gene in pGA482G 5) FLcpAS is the antisense form of the GFLV coat protein gene in pGA482G 6) FLcp + GUS is the GFLV sense translatable coat protein gene in pGA482GG 7) ritE2 del B is from Agrobacterium tumefiL- ciens in pBinl9 ~'From left to right: NPTII,
Biotechnology for a pesticide free Vineyard?
NR Rootstock CP gene GLRaV-2; GLRaV-3; GFLV Virus resistance 05/2005 05/2009 Niagara; Concord Drr206 Pisum sativum Fungal resistant 06/2004 06/2014 Chardonnay Thompson Seedless; Rootstock Saint George Polygalacturonase inhibitor protein P communis Fungal resistant 11/2007 09/2008 V vinifera Fungal resistant
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