qPCR Quantification Protocol Guide

• TaqMan ® Non-coding RNA Assays – Target long non-coding RNA (ncRNA) in human, mouse, and rat species These assays are designed to ncRNAs that are >60 nt in length This protocol provides instructions for real-time reverse transcription-PCR (real-time RT-PCR) using TaqMan Gene Expression Assays and TaqMan Non-coding RNA Assays

TaqMan One-Step RT-PCR Master Mix Reagents Kit Protocol P/N

TaqMan One-Step RT-PCR The TaqMan One-Step RT-PCR Reagents Kit provides a RT-PCR mix which may be used with any appropriately designed primer and probe set to detect total RNA or mRNA UNG cannot be used with one-step RT-PCR using the TaqMan One-Step RT-PCR Master Mix Reagents Kit UNG is active at the same

TaqMan® Gene Expression Assays Protocol (PN 4333458)

The following TaqMan® Gene Expression Assays are available: † Inventoried Assays – Predesigned real-time PCR assays that are previously manufactured and immediately available at the time you submit an order † Made-to-Order Assays – Predesigned real-time PCR assays that are manufactured at the time you submit an order

TaqMan Universal PCR Master Mix - Thermo Fisher Scientific

TaqMan ® Universal PCR Master Mix is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real‐time polymerase chain reaction (PCR) TaqMan ® Universal PCR Master Mix can be used to amplify

TaqMan Multiplex PCR Optimization

multiplex PCR with TaqMan ® Assays, and covers the following subjects: • Guidance on optimizing multiplex PCR reactions • Information on using products from Life Technologies to facilitate the performance of multiplex PCR and decrease the amount of time required to perform optimization

Quantitative Real Time PCR Protocol Stack Lab

dye and TaqMan® hydrolysis probe are the common examples for these two, respectively The SYBR Green method doesn’t need fluorescently labeled probe/primer and costs much less than the TaqMan method The protocol that follows is intended for use with the SYBR Green I method unless otherwise specified

A PCR QUANTITATIVE EN TEMPS REEL OU LA TAQMAN

- la technique de PCR quantitative par détection en temps réel de la fluorescence - l'appareil 7700 de chez Perkin Elmer (on dit alors plutôt " le TaqMan" ) Il est important de bien distinguer l' appareil de mesure du type de réaction chimique utilisé: Le mot "TaqMan" est dérivé des deux mots : Taq polymérase et de PacMan